The addition of human iPS cell-derived neural progenitors changes the contraction of human iPS cell-derived cardiac spheroids

Yosuke Mukae, Manabu Itoh, Ryo Noguchi, Kojiro Furukawa, Ken ichi Arai, Jun ichi Oyama, Shuji Toda, Koichi Nakayama, Koichi Node, Shigeki Morita*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

9 被引用数 (Scopus)

抄録

Background: We havebeen attempting to use cardiac spheroids to construct three-dimensional contractilestructures for failed hearts. Recent studies have reported that neuralprogenitors (NPs) play significant roles in heart regeneration. However, theeffect of NPs on the cardiac spheroid has not yet been elucidated. Objective: This studyaims to demonstrate the influence of NPs on the function of cardiac spheroids. Methods: Thespheroids were constructed on a low-attachment-well plate by mixing humaninduced pluripotent stem (hiPS) cell-derived cardiomyocytes and hiPScell-derived NPs (hiPS-NPs). The ratio of hiPS-NPs was set at 0%, 10%, 20%,30%, and 40% of the total cell number of spheroids, which was 2500. The motionwas recorded, and the fractional shortening and the contraction velocity weremeasured. Results: Spheroidswere formed within 48 h after mixing the cells, except for the spheroidscontaining 0% hiPS-NPs. Observation at day 7 revealed significant differencesin the fractional shortening (analysis of variance; p = 0.01). The bestfractional shortening was observed with the spheroids containing 30% hiPS-NPs.Neuronal cells were detected morphologically within the spheroids under aconfocal microscope. Conclusion: Theaddition of hiPS-NPs influenced the contractile function of the cardiacspheroids. Further studies are warranted to elucidate the underlying mechanism.

本文言語英語
ページ(範囲)61-67
ページ数7
ジャーナルTissue and Cell
53
DOI
出版ステータス出版済み - 2018/08

ASJC Scopus 主題領域

  • 発生生物学
  • 細胞生物学

フィンガープリント

「The addition of human iPS cell-derived neural progenitors changes the contraction of human iPS cell-derived cardiac spheroids」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル