Synthesis of novel caged antisense oligonucleotides with fluorescence property

Shin Hikage; Yasuo Sasaki; Terunobu Hisai; Hiroki Tanimoto; Tsumoru Morimoto; Yasuhiro Nishiyama; Kiyomi Kakiuchi

doi:10.1016/j.jphotochem.2016.01.007

Synthesis of novel caged antisense oligonucleotides with fluorescence property

Shin Hikage, Yasuo Sasaki, Terunobu Hisai, Hiroki Tanimoto, Tsumoru Morimoto, Yasuhiro Nishiyama^*, Kiyomi Kakiuchi

^*この論文の責任著者

生体認識化学研究室

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

8 被引用数 (Scopus)

抄録

In this study, novel caged antisense oligonucleotides with thiochromone S,S-dioxide as the photolabile protecting group were synthesized, and their photodeprotection was investigated. In vitro experiment, the original target molecule was successfully recovered by photoirradiation. Furthermore, the photodeprotection process was also successfully monitored by the fluorescence spectroscopy of highly fluorescent byproducts generated from the thiochromone-type photolabile protecting group.

本文言語	英語
ページ（範囲）	175-183
ページ数	9
ジャーナル	Journal of Photochemistry and Photobiology A: Chemistry
巻	331
DOI	https://doi.org/10.1016/j.jphotochem.2016.01.007
出版ステータス	出版済み - 2016/12/01

ASJC Scopus 主題領域

化学一般
化学工学一般
物理学および天文学一般

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10.1016/j.jphotochem.2016.01.007

引用スタイル

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title = "Synthesis of novel caged antisense oligonucleotides with fluorescence property",

abstract = "In this study, novel caged antisense oligonucleotides with thiochromone S,S-dioxide as the photolabile protecting group were synthesized, and their photodeprotection was investigated. In vitro experiment, the original target molecule was successfully recovered by photoirradiation. Furthermore, the photodeprotection process was also successfully monitored by the fluorescence spectroscopy of highly fluorescent byproducts generated from the thiochromone-type photolabile protecting group.",

keywords = "Antisense oligonucleotide, Caged compound, Fluorescence, Luciferin–luciferase reaction, Thiochromone",

author = "Shin Hikage and Yasuo Sasaki and Terunobu Hisai and Hiroki Tanimoto and Tsumoru Morimoto and Yasuhiro Nishiyama and Kiyomi Kakiuchi",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = dec,

day = "1",

doi = "10.1016/j.jphotochem.2016.01.007",

language = "英語",

volume = "331",

pages = "175--183",

journal = "Journal of Photochemistry and Photobiology A: Chemistry",

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TY - JOUR

T1 - Synthesis of novel caged antisense oligonucleotides with fluorescence property

AU - Hikage, Shin

AU - Sasaki, Yasuo

AU - Hisai, Terunobu

AU - Tanimoto, Hiroki

AU - Morimoto, Tsumoru

AU - Nishiyama, Yasuhiro

AU - Kakiuchi, Kiyomi

PY - 2016/12/1

Y1 - 2016/12/1

N2 - In this study, novel caged antisense oligonucleotides with thiochromone S,S-dioxide as the photolabile protecting group were synthesized, and their photodeprotection was investigated. In vitro experiment, the original target molecule was successfully recovered by photoirradiation. Furthermore, the photodeprotection process was also successfully monitored by the fluorescence spectroscopy of highly fluorescent byproducts generated from the thiochromone-type photolabile protecting group.

AB - In this study, novel caged antisense oligonucleotides with thiochromone S,S-dioxide as the photolabile protecting group were synthesized, and their photodeprotection was investigated. In vitro experiment, the original target molecule was successfully recovered by photoirradiation. Furthermore, the photodeprotection process was also successfully monitored by the fluorescence spectroscopy of highly fluorescent byproducts generated from the thiochromone-type photolabile protecting group.

KW - Antisense oligonucleotide

KW - Caged compound

KW - Fluorescence

KW - Luciferin–luciferase reaction

KW - Thiochromone

UR - http://www.scopus.com/inward/record.url?scp=84957381286&partnerID=8YFLogxK

U2 - 10.1016/j.jphotochem.2016.01.007

DO - 10.1016/j.jphotochem.2016.01.007

M3 - 学術論文

AN - SCOPUS:84957381286

SN - 1010-6030

VL - 331

SP - 175

EP - 183

JO - Journal of Photochemistry and Photobiology A: Chemistry

JF - Journal of Photochemistry and Photobiology A: Chemistry

ER -

Synthesis of novel caged antisense oligonucleotides with fluorescence property

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ASJC Scopus 主題領域

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