TY - JOUR
T1 - Survivin inhibits apoptosis in cytotrophoblasts
AU - Shiozaki, A.
AU - Kataoka, K.
AU - Fujimura, M.
AU - Yuki, H.
AU - Sakai, M.
AU - Saito, S.
N1 - Funding Information:
We would like to thank Dr Nam-ho Huh and Dr Mikiro Takaishi for helpful discussion. We gratefully thank Dr Yoshihiro Yamakawa, Ms Keiko Maeda, Mr Sadaaki Yamamoto, and Ms Yuki Kunioku for technical assistance. We also thank Ms Reiko Yasukawa for careful preparation of this manuscript. This work was supported in part by a grant from the Ministry of Education, Science and Culture of Japan (13470347).
PY - 2003/1
Y1 - 2003/1
N2 - Survivin, a protein that inhibits apoptosis, is expressed in a variety of tumour cells. We detected survivin-specific mRNA and protein in normal placental tissues, two human choriocarcinoma cell lines (JEG-3 and BeWo), and a trophoblastic cell line (tPA30-1) by reverse transcription-polymerase chain reaction (RT-PCR), Northern blotting, and Western blotting. Immunohistochemically, survivin was localized to normal villous cytotrophoblasts, normal extravillous trophoblasts, cytotrophoblasts in hydatidiform mole, and choriocarcinoma cells. Antisense oligonucleotides for survivin dose-dependently induced apoptosis in two choriocarcinoma cell lines (JEG-3 and BeWo) and a trophoblastic cell line (tPA30-1), while sense oligonucleotides showed little effect. These findings suggest that survivin antagonizes apoptosis in cytotrophoblasts, extravillous trophoblasts, and choriocarcinoma cells.
AB - Survivin, a protein that inhibits apoptosis, is expressed in a variety of tumour cells. We detected survivin-specific mRNA and protein in normal placental tissues, two human choriocarcinoma cell lines (JEG-3 and BeWo), and a trophoblastic cell line (tPA30-1) by reverse transcription-polymerase chain reaction (RT-PCR), Northern blotting, and Western blotting. Immunohistochemically, survivin was localized to normal villous cytotrophoblasts, normal extravillous trophoblasts, cytotrophoblasts in hydatidiform mole, and choriocarcinoma cells. Antisense oligonucleotides for survivin dose-dependently induced apoptosis in two choriocarcinoma cell lines (JEG-3 and BeWo) and a trophoblastic cell line (tPA30-1), while sense oligonucleotides showed little effect. These findings suggest that survivin antagonizes apoptosis in cytotrophoblasts, extravillous trophoblasts, and choriocarcinoma cells.
UR - http://www.scopus.com/inward/record.url?scp=0038303450&partnerID=8YFLogxK
U2 - 10.1053/plac.2002.0860
DO - 10.1053/plac.2002.0860
M3 - 学術論文
C2 - 12495661
AN - SCOPUS:0038303450
SN - 0143-4004
VL - 24
SP - 65
EP - 76
JO - Placenta
JF - Placenta
IS - 1
ER -