@article{d9e7f2b6cc2f428c91aca0cf2fcbf8fc,
title = "Solid-state NMR 13C and 15N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin",
abstract = "Anabaena Sensory Rhodopsin (ASR) is a unique microbial rhodopsin that displays photocromism, interacts with soluble transducer, and may be involved in gene regulation. Here we report nearly complete spectroscopic 13C and 15N assignments of ASR reconstituted in lipids, obtained using two- and three-dimensional magic angle spinning solid state NMR spectroscopy on alternately 13C labeled samples. The obtained chemical shifts are used to characterize the protein backbone conformation. They suggest that lipid-reconstituted ASR has a fold generally similar to that seen in earlier X-ray studies, but with a number of important differences. SSNMR detects double conformations for a number of residues on the cytoplasmic side.",
keywords = "Anabaena Sensory Rhodopsin, Bacteriorhodopsin, Chemical shifts, Magic-angle spinning, Secondary structure, Solid-state NMR",
author = "Shenlin Wang and Lichi Shi and Takashi Okitsu and Akimori Wada and Brown, {Leonid S.} and Vladimir Ladizhansky",
note = "Funding Information: Acknowledgments This research was supported by Natural Science and Engineering Research Council of Canada, Canada Foundation of Innovation and Ontario Ministry of Research and Innovation. S.W. is supported by the Canadian Institute of Health Research postdoctoral Fellowship. V.L. holds Canada Research Chair in Biophysics. We thank Prof. Kwang-Hwan Jung (Sogang University, Seoul, Korea) for the generous gift of a plasmid encoding C-terminally 69 His-tagged ASR.",
year = "2013",
month = oct,
doi = "10.1007/s12104-012-9421-y",
language = "英語",
volume = "7",
pages = "253--256",
journal = "Biomolecular NMR Assignments",
issn = "1874-2718",
publisher = "Springer Science and Business Media B.V.",
number = "2",
}