Simultaneous Determination of Ethyleneamines by Reversed-Phase Ion-Pair Chromatography with Ultraviolet Detection Using on-Line Complexation with Copper(II) Ion

Azusa Yudo*, Tomoko Kemmei, Shuji Kodama, Yoshinori Inoue, Shigehiro Kagaya

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

1 被引用数 (Scopus)

抄録

A selective and simple method was developed for the determination of four ethyleneamines (EAs), i.e., ethylenediamine (EDA), diethylenetriamine (DETA), triethylenetetramine (TETA), and tetraethylenepentamine (TEPA), by reversed-phase ion-pair chromatography with ultraviolet detection. The method is based on the on-line complexation of EAs with a Cu(II) ion added to the mobile phase. The Cu(II) complexes with EAs (Cu(II)-EA complexes) were well separated on a reversed-phased column, using 1-octanesulfonate as an ion-pairing reagent. The concentrations of 1-octanesulfonate and acetonitrile in the mobile phase significantly influenced the retention times of EAs. The high concentration of the Cu(II) ion in the mobile phase resulted in the increase of the baseline noise. A mixture of acetonitrile and 50 mM acetate buffer at pH 5.5 (23:77, v/v) which contains 0.5 mM CuSO4 and 20 mM 1-octanesulfonic acid sodium salt was used as the mobile phase. Cu(II)-EA complexes could be detected at 243 nm. The limits of quantification of EAs (S/N = 10) were 0.003 mM for EDA, DETA, and TETA and 0.01 mM for TEPA. The calibration curves were linear over three orders of magnitude of EA concentrations with high correlation coefficients (r2 = 1.000). The proposed method was successfully applied to determine EAs in epoxy resin curing agents.

本文言語英語
ページ(範囲)463-470
ページ数8
ジャーナルChromatographia
87
7-8
DOI
出版ステータス出版済み - 2024/08

ASJC Scopus 主題領域

  • 分析化学
  • 生化学
  • 臨床生化学
  • 有機化学

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