Severity of acute lung injury in aspiration pneumonia model mice transplanted with human oral microflora

Manami Hayashi, Mina Mori, Momoe Itsumi, Natsuno Nakamura, Takashi Takaki, Kaoru Onoyama, Haruka Fukamachi, Mie Kurosawa, Hirobumi Morisaki, Takahiro Funatsu, Mie Myers, Takehiko Iijima, Hirotaka Kuwata*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

1 被引用数 (Scopus)

抄録

Objectives: When the microflora balance becomes disturbed, referred to as “dysbiosis,” immune homeostasis is altered, triggering various diseases. In the oral cavity, it is also becoming evident that dysbiosis can act as a stimulus for various systemic diseases. Aspiration pneumonia occurs when oral microflora enters the lungs of elderly individuals. Aspiration pneumonia is highly lethal and intractable; thus, a detailed understanding of the interaction between the microflora and lung immunity is essential to establish the optimal treatment. Methods: Human oral microflora-associated mice (HOMA) were created by transplanting human oral commensal microflora into germ-free mice. Then, the effects of oral microflora were analyzed on lung immunity and systemic conditions of acute lung injury and acute respiratory distress syndrome (ALI/ARDS). Results: The microflora in HOMA was in a state of dysbiosis. Furthermore, the accumulation of neutrophils in the lungs during septic conditions was more significant in HOMA. Serum tumor necrosis factor alpha (TNF-α) increased considerably in HOMA during ALI/ARDS. Simultaneously, the tissue distribution of lung macrophages in HOMA decreased more significantly in alveolar macrophages than in tissue macrophages. Finally, we found that microbial compositions in the lungs during ALI/ARDS were only a single or a small number of species, as in aspiration pneumonia in humans. Conclusion: Systemic inflammation and lung tissue damage were observed more significantly in HOMA during ALI/ARDS, suggesting that dysbiosis has a synergistic effect with septic shock, resulting in severe injury. Thus, oral and systemic dysbiosis may be a potential target for treating aspiration pneumonia in the elderly.

本文言語英語
ページ(範囲)121-130
ページ数10
ジャーナルOral Science International
21
1
DOI
出版ステータス出版済み - 2024/01

ASJC Scopus 主題領域

  • 耳鼻咽喉科学

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