Separation of inorganic anions on reversed-phase C18 columns with a phosphomolybdate mobile phase

Tomoko Kemmei*, Azusa Yudo, Shuji Kodama, Atsushi Yamamoto, Yoshinori Inoue, Shigehiro Kagaya, Kazuichi Hayakawa

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

1 被引用数 (Scopus)

抄録

Reversed-phase high performance liquid chromatography (RP-HPLC) is the most widely used chromatographic method. In addition to hydrophobic interactions, additional interactions such as electrostatic interactions may participate in the retention behaviour of an analyte. This makes it possible to use RP-HPLC for many types of analyte. We describe a simple method for separating inorganic anions on a C18 column, in which retention of inorganic anions is almost entirely due to electrostatic interactions. This leads to rapid separations as well as higher theoretical plate numbers. We used 2 mM phosphoric acid containing a low concentration of disodium molybdate as the mobile phase, which allows UV detection of non-UV-absorbing anions. With this method, we determined eight inorganic anions including several non-UV-absorbing anions photometrically at 220 nm. The detection limits of the examined eight inorganic anions calculated at a signal-to-noise ratio of 3 were between 0.3 and 10 μM. The detector response was linear over three orders of magnitude of inorganic anion concentration. The proposed RP-HPLC/UV method was successfully applied to determine inorganic anions in some water samples.

本文言語英語
論文番号464843
ジャーナルJournal of Chromatography A
1722
DOI
出版ステータス出版済み - 2024/05/10

ASJC Scopus 主題領域

  • 分析化学
  • 生化学
  • 有機化学

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