TY - JOUR
T1 - Replacement of Rat Tracheas by Layered, Trachea-Like, Scaffold-Free Structures of Human Cells Using a Bio-3D Printing System
AU - Machino, Ryusuke
AU - Matsumoto, Keitaro
AU - Taniguchi, Daisuke
AU - Tsuchiya, Tomoshi
AU - Takeoka, Yosuke
AU - Taura, Yasuaki
AU - Moriyama, Masaaki
AU - Tetsuo, Tomoyuki
AU - Oyama, Shosaburo
AU - Takagi, Katsunori
AU - Miyazaki, Takuro
AU - Hatachi, Go
AU - Doi, Ryoichiro
AU - Shimoyama, Koichiro
AU - Matsuo, Naoto
AU - Yamasaki, Naoya
AU - Nakayama, Koichi
AU - Nagayasu, Takeshi
N1 - Publisher Copyright:
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/4/11
Y1 - 2019/4/11
N2 - Current scaffold-based tissue engineering approaches are subject to several limitations, such as design inflexibility, poor cytocompatibility, toxicity, and post-transplant degradation. Thus, scaffold-free tissue-engineered structures can be a promising solution to overcome the issues associated with classical scaffold-based materials in clinical transplantation. The present study seeks to optimize the culture conditions and cell combinations used to generate scaffold-free structures using a Bio-3D printing system. Human cartilage cells, human fibroblasts, human umbilical vein endothelial cells, and human mesenchymal stem cells from bone marrow are aggregated into spheroids and placed into a Bio-3D printing system with dedicated needles positioned according to 3D configuration data, to develop scaffold-free trachea-like tubes. Culturing the Bio-3D-printed structures with proper flow of specific medium in a bioreactor facilitates the rearrangement and self-organization of cells, improving physical strength and tissue function. The Bio-3D-printed tissue forms small-diameter trachea-like tubes that are implanted into rats with the support of catheters. It is confirmed that the tubes are viable in vivo and that the tracheal epithelium and capillaries proliferate. This tissue-engineered, scaffold-free, tubular structure can represent a significant step toward clinical application of bioengineered organs.
AB - Current scaffold-based tissue engineering approaches are subject to several limitations, such as design inflexibility, poor cytocompatibility, toxicity, and post-transplant degradation. Thus, scaffold-free tissue-engineered structures can be a promising solution to overcome the issues associated with classical scaffold-based materials in clinical transplantation. The present study seeks to optimize the culture conditions and cell combinations used to generate scaffold-free structures using a Bio-3D printing system. Human cartilage cells, human fibroblasts, human umbilical vein endothelial cells, and human mesenchymal stem cells from bone marrow are aggregated into spheroids and placed into a Bio-3D printing system with dedicated needles positioned according to 3D configuration data, to develop scaffold-free trachea-like tubes. Culturing the Bio-3D-printed structures with proper flow of specific medium in a bioreactor facilitates the rearrangement and self-organization of cells, improving physical strength and tissue function. The Bio-3D-printed tissue forms small-diameter trachea-like tubes that are implanted into rats with the support of catheters. It is confirmed that the tubes are viable in vivo and that the tracheal epithelium and capillaries proliferate. This tissue-engineered, scaffold-free, tubular structure can represent a significant step toward clinical application of bioengineered organs.
KW - bio-3D printers
KW - bioengineered organs
KW - scaffold-free
KW - tissue engineering
KW - tracheal regeneration
UR - http://www.scopus.com/inward/record.url?scp=85059904278&partnerID=8YFLogxK
U2 - 10.1002/adhm.201800983
DO - 10.1002/adhm.201800983
M3 - 学術論文
C2 - 30632706
AN - SCOPUS:85059904278
SN - 2192-2640
VL - 8
JO - Advanced Healthcare Materials
JF - Advanced Healthcare Materials
IS - 7
M1 - 1800983
ER -