Replacement of Rat Tracheas by Layered, Trachea-Like, Scaffold-Free Structures of Human Cells Using a Bio-3D Printing System

Ryusuke Machino, Keitaro Matsumoto, Daisuke Taniguchi, Tomoshi Tsuchiya, Yosuke Takeoka, Yasuaki Taura, Masaaki Moriyama, Tomoyuki Tetsuo, Shosaburo Oyama, Katsunori Takagi, Takuro Miyazaki, Go Hatachi, Ryoichiro Doi, Koichiro Shimoyama, Naoto Matsuo, Naoya Yamasaki, Koichi Nakayama, Takeshi Nagayasu*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

71 被引用数 (Scopus)

抄録

Current scaffold-based tissue engineering approaches are subject to several limitations, such as design inflexibility, poor cytocompatibility, toxicity, and post-transplant degradation. Thus, scaffold-free tissue-engineered structures can be a promising solution to overcome the issues associated with classical scaffold-based materials in clinical transplantation. The present study seeks to optimize the culture conditions and cell combinations used to generate scaffold-free structures using a Bio-3D printing system. Human cartilage cells, human fibroblasts, human umbilical vein endothelial cells, and human mesenchymal stem cells from bone marrow are aggregated into spheroids and placed into a Bio-3D printing system with dedicated needles positioned according to 3D configuration data, to develop scaffold-free trachea-like tubes. Culturing the Bio-3D-printed structures with proper flow of specific medium in a bioreactor facilitates the rearrangement and self-organization of cells, improving physical strength and tissue function. The Bio-3D-printed tissue forms small-diameter trachea-like tubes that are implanted into rats with the support of catheters. It is confirmed that the tubes are viable in vivo and that the tracheal epithelium and capillaries proliferate. This tissue-engineered, scaffold-free, tubular structure can represent a significant step toward clinical application of bioengineered organs.

本文言語英語
論文番号1800983
ジャーナルAdvanced Healthcare Materials
8
7
DOI
出版ステータス出版済み - 2019/04/11

ASJC Scopus 主題領域

  • 生体材料
  • 生体医工学
  • 薬科学

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