Rat pancreas contains the proglucagon(64-69) fragment and arginine stimulates its release

Chizuko Yanaihara; Tomoaki Matsumoto; Makoto Kadowaki; Kazuaki Iguchi; Noboru Yanaihara

doi:10.1016/0014-5793(85)81265-5

Rat pancreas contains the proglucagon(64-69) fragment and arginine stimulates its release

Chizuko Yanaihara, Tomoaki Matsumoto, Makoto Kadowaki, Kazuaki Iguchi, Noboru Yanaihara^*

^*この論文の責任著者

未病研究センター

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

12 被引用数 (Scopus)

抄録

Rat proglucagon(64-69) corresponding to the C-terminal hexapeptide of putative rat glicentin sequence in the precursor was synthesized. A glicentin C-terminal hexapeptide specific radioimmunoassay, using the synthetic hexapeptide as standard, demonstrated the presence in rat pancreas of a peptide identified with the synthetic rat proglucagon(64-69): H-Asn-Arg-Asn-Asn-Ile-Ala-OH. The hexapeptide was released concomitantly with glucagon by arginine stimulation from the isolated perfused rat pancreas. The results indicate that the pancreas co-stores and possibly co-releases the hexapeptide with glucagon as one of the processing products of proglucagon.

本文言語	英語
ページ（範囲）	307-310
ページ数	4
ジャーナル	FEBS Letters
巻	187
号	2
DOI	https://doi.org/10.1016/0014-5793(85)81265-5
出版ステータス	出版済み - 1985/08/05

ASJC Scopus 主題領域

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/0014-5793(85)81265-5

引用スタイル

@article{fda5a29f3b9e4f848b2ec8cc9fd1beec,

title = "Rat pancreas contains the proglucagon(64-69) fragment and arginine stimulates its release",

abstract = "Rat proglucagon(64-69) corresponding to the C-terminal hexapeptide of putative rat glicentin sequence in the precursor was synthesized. A glicentin C-terminal hexapeptide specific radioimmunoassay, using the synthetic hexapeptide as standard, demonstrated the presence in rat pancreas of a peptide identified with the synthetic rat proglucagon(64-69): H-Asn-Arg-Asn-Asn-Ile-Ala-OH. The hexapeptide was released concomitantly with glucagon by arginine stimulation from the isolated perfused rat pancreas. The results indicate that the pancreas co-stores and possibly co-releases the hexapeptide with glucagon as one of the processing products of proglucagon.",

keywords = "Arginine-stimulated glucagon release, Glicentin (64-69), Isolated rat pancreas perfusion, Peptide synthesis, Radioimmunoassay, Rat proglucagon",

author = "Chizuko Yanaihara and Tomoaki Matsumoto and Makoto Kadowaki and Kazuaki Iguchi and Noboru Yanaihara",

note = "Funding Information: This work was supportedi n part by Grant-in-Aid for Scientific Researchn o.59227005fr om the Ministry of Education and for Cancer Research no.56-6f rom the Ministry of Health and Welfare, Japan.",

year = "1985",

month = aug,

day = "5",

doi = "10.1016/0014-5793(85)81265-5",

language = "英語",

volume = "187",

pages = "307--310",

journal = "FEBS Letters",

issn = "0014-5793",

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TY - JOUR

T1 - Rat pancreas contains the proglucagon(64-69) fragment and arginine stimulates its release

AU - Yanaihara, Chizuko

AU - Matsumoto, Tomoaki

AU - Kadowaki, Makoto

AU - Iguchi, Kazuaki

AU - Yanaihara, Noboru

N1 - Funding Information: This work was supportedi n part by Grant-in-Aid for Scientific Researchn o.59227005fr om the Ministry of Education and for Cancer Research no.56-6f rom the Ministry of Health and Welfare, Japan.

PY - 1985/8/5

Y1 - 1985/8/5

N2 - Rat proglucagon(64-69) corresponding to the C-terminal hexapeptide of putative rat glicentin sequence in the precursor was synthesized. A glicentin C-terminal hexapeptide specific radioimmunoassay, using the synthetic hexapeptide as standard, demonstrated the presence in rat pancreas of a peptide identified with the synthetic rat proglucagon(64-69): H-Asn-Arg-Asn-Asn-Ile-Ala-OH. The hexapeptide was released concomitantly with glucagon by arginine stimulation from the isolated perfused rat pancreas. The results indicate that the pancreas co-stores and possibly co-releases the hexapeptide with glucagon as one of the processing products of proglucagon.

AB - Rat proglucagon(64-69) corresponding to the C-terminal hexapeptide of putative rat glicentin sequence in the precursor was synthesized. A glicentin C-terminal hexapeptide specific radioimmunoassay, using the synthetic hexapeptide as standard, demonstrated the presence in rat pancreas of a peptide identified with the synthetic rat proglucagon(64-69): H-Asn-Arg-Asn-Asn-Ile-Ala-OH. The hexapeptide was released concomitantly with glucagon by arginine stimulation from the isolated perfused rat pancreas. The results indicate that the pancreas co-stores and possibly co-releases the hexapeptide with glucagon as one of the processing products of proglucagon.

KW - Arginine-stimulated glucagon release

KW - Glicentin (64-69)

KW - Isolated rat pancreas perfusion

KW - Peptide synthesis

KW - Radioimmunoassay

KW - Rat proglucagon

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U2 - 10.1016/0014-5793(85)81265-5

DO - 10.1016/0014-5793(85)81265-5

M3 - 学術論文

C2 - 4018268

AN - SCOPUS:0021968921

SN - 0014-5793

VL - 187

SP - 307

EP - 310

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Rat pancreas contains the proglucagon(64-69) fragment and arginine stimulates its release

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