抄録
Tropomyosin-specific photoaffinity adenosine triphosphate (ATP) probes have been first developed, in which a diazirine moiety is incorporated into the γ-phosphate group as a rapidly carbene-generating photophore. These probes clearly labeled tropomyosin in the presence of other actomyosin components, that is, myosin, actin, and troponins. The specific labeling of tropomyosin was easily identified by selective trapping of the photo-incorporated ATP probe on Fe3+-immobilized metal ion affinity chromatography (IMAC) beads. The characteristic nature of tropomyosin-specific photocross-linking was further confirmed with a biotin-carrying derivative of the ATP probe. These data suggest that the tropomyosin on the actin filament assembly is located in close proximity to the ATP binding cavity of myosin.
本文言語 | 英語 |
---|---|
ページ(範囲) | 2252-2254 |
ページ数 | 3 |
ジャーナル | Bioorganic and Medicinal Chemistry Letters |
巻 | 21 |
号 | 8 |
DOI | |
出版ステータス | 出版済み - 2011/04/15 |
ASJC Scopus 主題領域
- 生化学
- 分子医療
- 分子生物学
- 薬科学
- 創薬
- 臨床生化学
- 有機化学