TY - JOUR
T1 - Rapid detection of leukemia cells by use of a complement-mediated cytolytic reaction and imaging sensor system
AU - Suzuki, M.
AU - Tamiya, E.
AU - Kataoka, T.
AU - Tokunaga, T.
AU - Karube, I.
PY - 1987
Y1 - 1987
N2 - We describe a system for detection of leukemia cells involving complement-mediated cytotoxic reaction and an image processing system, consisting of a charge-coupled-device image sensor, an image memory board, a personal computer, and a phase-contrast microscope. Then added to a cell suspension, monoclonal antibody specific to the fetal thymus antigen-1 of the mouse leukemia GRSL cell produced cytolysis of only GRSL cells. This cytolysis decreased the brightness of the cells observed by phase-contrast microscopy. The remaining brightness was subtracted from that of the phase-contrast image of the cells before cytolysis, which had been converted to a digital signal and stored in computer memory. Measurement time is 2 s. The time course for complete GRSL cytolysis, as measured with this system, is 12 min; overall measurement time, including reaction time, is approximately 15 min. GRSL cells in a suspension of mixed cells were determined specifically by the system.
AB - We describe a system for detection of leukemia cells involving complement-mediated cytotoxic reaction and an image processing system, consisting of a charge-coupled-device image sensor, an image memory board, a personal computer, and a phase-contrast microscope. Then added to a cell suspension, monoclonal antibody specific to the fetal thymus antigen-1 of the mouse leukemia GRSL cell produced cytolysis of only GRSL cells. This cytolysis decreased the brightness of the cells observed by phase-contrast microscopy. The remaining brightness was subtracted from that of the phase-contrast image of the cells before cytolysis, which had been converted to a digital signal and stored in computer memory. Measurement time is 2 s. The time course for complete GRSL cytolysis, as measured with this system, is 12 min; overall measurement time, including reaction time, is approximately 15 min. GRSL cells in a suspension of mixed cells were determined specifically by the system.
UR - http://www.scopus.com/inward/record.url?scp=0023204961&partnerID=8YFLogxK
U2 - 10.1093/clinchem/33.4.558
DO - 10.1093/clinchem/33.4.558
M3 - 学術論文
C2 - 3829390
AN - SCOPUS:0023204961
SN - 0009-9147
VL - 33
SP - 558
EP - 561
JO - Clinical Chemistry
JF - Clinical Chemistry
IS - 4
ER -