TY - JOUR
T1 - Quantitative Threshold Determination of Auditory Brainstem Responses in Mouse Models
AU - Tanaka, Kenji
AU - Ohara, Shuma
AU - Matsuzaka, Tadaaki
AU - Matsugaki, Aira
AU - Ishimoto, Takuya
AU - Ozasa, Ryosuke
AU - Kuroda, Yukiko
AU - Matsuo, Koichi
AU - Nakano, Takayoshi
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/7
Y1 - 2023/7
N2 - The auditory brainstem response (ABR) is a scalp recording of potentials produced by sound stimulation, and is commonly used as an indicator of auditory function. However, the ABR threshold, which is the lowest audible sound pressure, cannot be objectively determined since it is determined visually using a measurer, and this has been a problem for several decades. Although various algorithms have been developed to objectively determine ABR thresholds, they remain lacking in terms of accuracy, efficiency, and convenience. Accordingly, we proposed an improved algorithm based on the mutual covariance at adjacent sound pressure levels. An ideal ABR waveform with clearly defined waves I–V was created; moreover, using this waveform as a standard template, the experimentally obtained ABR waveform was inspected for disturbances based on mutual covariance. The ABR testing was repeated if the value was below the established cross-covariance reference value. Our proposed method allowed more efficient objective determination of ABR thresholds and a smaller burden on experimental animals.
AB - The auditory brainstem response (ABR) is a scalp recording of potentials produced by sound stimulation, and is commonly used as an indicator of auditory function. However, the ABR threshold, which is the lowest audible sound pressure, cannot be objectively determined since it is determined visually using a measurer, and this has been a problem for several decades. Although various algorithms have been developed to objectively determine ABR thresholds, they remain lacking in terms of accuracy, efficiency, and convenience. Accordingly, we proposed an improved algorithm based on the mutual covariance at adjacent sound pressure levels. An ideal ABR waveform with clearly defined waves I–V was created; moreover, using this waveform as a standard template, the experimentally obtained ABR waveform was inspected for disturbances based on mutual covariance. The ABR testing was repeated if the value was below the established cross-covariance reference value. Our proposed method allowed more efficient objective determination of ABR thresholds and a smaller burden on experimental animals.
KW - ABR threshold
KW - auditory brainstem responses (ABR)
KW - auditory function
KW - correlation coefficient
KW - cross-covariance
UR - http://www.scopus.com/inward/record.url?scp=85166015015&partnerID=8YFLogxK
U2 - 10.3390/ijms241411393
DO - 10.3390/ijms241411393
M3 - 学術論文
C2 - 37511152
AN - SCOPUS:85166015015
SN - 1661-6596
VL - 24
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 14
M1 - 11393
ER -