Quality assessment of Rheum species cultivated in Japan by focusing on M2 polarization of microglia

Tomoharu Kuboyama*, Kenichiro Hotta, Mai Asanuma, Yue Wei Ge, Kazufumi Toume, Takuma Yamazaki, Katsuko Komatsu*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

2 被引用数 (Scopus)

抄録

In traditional Japanese medicine, Rhei Rhizoma is used as a purgative, blood stasis-resolving and antipsychotic drug. The latter two properties are possibly related to anti-inflammatory effects. Microglia regulate inflammation in the central nervous system. M1 microglia induce inflammation, while M2 microglia inhibit inflammation and show neurotrophic effects. This study investigated the effects from water extracts of roots of cultivated Rheum species in Nagano Prefecture, Japan (strain C, a related strain to a Japanese cultivar, ‘Shinshu-Daio’; and strain 29, a Chinese strain) and 3 kinds of Rhei Rhizoma available in the Japanese market, and also examined their constituents on the polarization of cultured microglia. All extracts significantly decreased M1 microglia, and strains C and 29 significantly increased M2 microglia. Furthermore, the extracts of both strains significantly increased the M2/M1 ratio. Among the constituents of Rhei Rhizoma, (+)-catechin (2), resveratrol 4′-O-β-D-(6″-O-galloyl) glucopyranoside (5), isolindleyin (8), and physcion (15) significantly increased the M2/M1 ratio. The contents of the constituents in water extract of each strain were quantified using HPLC. The extracts of strains C and 29 contained relatively large amounts of 2 and 5; and 2, 8, and 15, respectively. This study showed the water extracts of roots of cultivated Rheum strains in Japan had the effects of M2 polarization of microglia, suggesting that these strains become the candidate to develop anti-inflammatory Rhei Rhizoma. Moreover, the suitable chemical composition to possess anti-inflammatory activity in the brain was clarified for the future development of new type of Rhei Rhizoma. Graphical Abstract: [Figure not available: see fulltext.]

本文言語英語
ページ(範囲)699-711
ページ数13
ジャーナルNatural Medicines
77
4
DOI
出版ステータス出版済み - 2023/09

ASJC Scopus 主題領域

  • 薬科学
  • 創薬
  • 補完代替医療
  • 有機化学

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