Protective behavior of tea catechins against DNA double strand breaks produced by radiations with different linear energy transfer

Takuro Wada; Ayaka Koike; Shota Yamazaki; Kyosuke Ashizawa; Fei Sun; Yuji Hatano; Hiroto Shimoyachi; Takahiro Kenmotsu; Takashi Ikka; Yasuhisa Oya

doi:10.1016/j.fusengdes.2021.112700

Protective behavior of tea catechins against DNA double strand breaks produced by radiations with different linear energy transfer

Takuro Wada, Ayaka Koike, Shota Yamazaki, Kyosuke Ashizawa, Fei Sun, Yuji Hatano, Hiroto Shimoyachi, Takahiro Kenmotsu, Takashi Ikka, Yasuhisa Oya^*

^*この論文の責任著者

研究推進機構水素同位体科学研究センター

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

2 被引用数 (Scopus)

抄録

The number of DNA double strand breaks (DSBs) of genome-sized DNA was quantitatively evaluated under several types of radiation sources. The characteristics of radiation damages by different linear energy transfer (LET) and the effect of radiation protection by tea catechins were also studied as a function of its concentration. After β-rays and γ-rays irradiation to samples, the length of genome size DNA molecules (bacteriophage T4 GT7 DNA; 166 kbp) was measured by single molecule observation method using fluorescence microscope, which can estimate DSBs quantitatively. It was found that the number of DSBs was increased with increasing LET due to high radical density. By addition of EGCg ((-)-epigallocatechin gallate), the number of DSBs was reduced with a small concentration of 1 µM.

本文言語	英語
論文番号	112700
ジャーナル	Fusion Engineering and Design
巻	172
DOI	https://doi.org/10.1016/j.fusengdes.2021.112700
出版ステータス	出版済み - 2021/11

ASJC Scopus 主題領域

土木構造工学
原子力エネルギーおよび原子力工学
材料科学一般
機械工学

文献へのアクセス

10.1016/j.fusengdes.2021.112700

フィンガープリント

「Protective behavior of tea catechins against DNA double strand breaks produced by radiations with different linear energy transfer」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

@article{ed6ca377c0124006aea7241e537066c5,

title = "Protective behavior of tea catechins against DNA double strand breaks produced by radiations with different linear energy transfer",

abstract = "The number of DNA double strand breaks (DSBs) of genome-sized DNA was quantitatively evaluated under several types of radiation sources. The characteristics of radiation damages by different linear energy transfer (LET) and the effect of radiation protection by tea catechins were also studied as a function of its concentration. After β-rays and γ-rays irradiation to samples, the length of genome size DNA molecules (bacteriophage T4 GT7 DNA; 166 kbp) was measured by single molecule observation method using fluorescence microscope, which can estimate DSBs quantitatively. It was found that the number of DSBs was increased with increasing LET due to high radical density. By addition of EGCg ((-)-epigallocatechin gallate), the number of DSBs was reduced with a small concentration of 1 µM.",

keywords = "DNA damage, Fluorescence microscope, Tritium",

author = "Takuro Wada and Ayaka Koike and Shota Yamazaki and Kyosuke Ashizawa and Fei Sun and Yuji Hatano and Hiroto Shimoyachi and Takahiro Kenmotsu and Takashi Ikka and Yasuhisa Oya",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = nov,

doi = "10.1016/j.fusengdes.2021.112700",

language = "英語",

volume = "172",

journal = "Fusion Engineering and Design",

issn = "0920-3796",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Protective behavior of tea catechins against DNA double strand breaks produced by radiations with different linear energy transfer

AU - Wada, Takuro

AU - Koike, Ayaka

AU - Yamazaki, Shota

AU - Ashizawa, Kyosuke

AU - Sun, Fei

AU - Hatano, Yuji

AU - Shimoyachi, Hiroto

AU - Kenmotsu, Takahiro

AU - Ikka, Takashi

AU - Oya, Yasuhisa

PY - 2021/11

Y1 - 2021/11

N2 - The number of DNA double strand breaks (DSBs) of genome-sized DNA was quantitatively evaluated under several types of radiation sources. The characteristics of radiation damages by different linear energy transfer (LET) and the effect of radiation protection by tea catechins were also studied as a function of its concentration. After β-rays and γ-rays irradiation to samples, the length of genome size DNA molecules (bacteriophage T4 GT7 DNA; 166 kbp) was measured by single molecule observation method using fluorescence microscope, which can estimate DSBs quantitatively. It was found that the number of DSBs was increased with increasing LET due to high radical density. By addition of EGCg ((-)-epigallocatechin gallate), the number of DSBs was reduced with a small concentration of 1 µM.

AB - The number of DNA double strand breaks (DSBs) of genome-sized DNA was quantitatively evaluated under several types of radiation sources. The characteristics of radiation damages by different linear energy transfer (LET) and the effect of radiation protection by tea catechins were also studied as a function of its concentration. After β-rays and γ-rays irradiation to samples, the length of genome size DNA molecules (bacteriophage T4 GT7 DNA; 166 kbp) was measured by single molecule observation method using fluorescence microscope, which can estimate DSBs quantitatively. It was found that the number of DSBs was increased with increasing LET due to high radical density. By addition of EGCg ((-)-epigallocatechin gallate), the number of DSBs was reduced with a small concentration of 1 µM.

KW - DNA damage

KW - Fluorescence microscope

KW - Tritium

UR - http://www.scopus.com/inward/record.url?scp=85108837872&partnerID=8YFLogxK

U2 - 10.1016/j.fusengdes.2021.112700

DO - 10.1016/j.fusengdes.2021.112700

M3 - 学術論文

AN - SCOPUS:85108837872

SN - 0920-3796

VL - 172

JO - Fusion Engineering and Design

JF - Fusion Engineering and Design

M1 - 112700

ER -