Plant lanosterol synthase: Divergence of the sterol and triterpene biosynthetic pathways in eukaryotes

Satoru Sawai, Tomoyoshi Akashi, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata, Shin Ichi Ayabe, Toshio Aoki*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

57 被引用数 (Scopus)

抄録

Sterols, essential eukaryotic constituents, are biosynthesized through either cyclic triterpenes, lanosterol (fungi and animals) or cycloartenol (plants). The cDNA for OSC7 of Lotus japonicus was shown to encode lanosterol synthase (LAS) by the complementation of a LAS-deficient mutant yeast and structural identification of the accumulated lanosterol. A double site-directed mutant of OSC7, in which amino acid residues crucial for the reaction specificity were changed to the cycloartenol synthase (CAS) type, produced parkeol and cycloartenol. The multiple amino acid sequence alignment of a conserved region suggests that the LAS of different eukaryotic lineages emerged from the ancestral CAS by convergent evolution. JSPP

本文言語英語
ページ(範囲)673-677
ページ数5
ジャーナルPlant and Cell Physiology
47
5
DOI
出版ステータス出版済み - 2006/05

ASJC Scopus 主題領域

  • 生理学
  • 植物科学
  • 細胞生物学

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