Pathophysiological roles of neuro-immune interactions between enteric neurons and mucosal mast cells in the gut of food allergy mice

Tomoe Yashiro, Hanako Ogata, Syed Faisal Zaidi, Jaemin Lee, Shusaku Hayashi, Takashi Yamamoto, Makoto Kadowaki*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

15 被引用数 (Scopus)

抄録

Recently, the involvement of the nervous system in the pathology of allergic diseases has attracted increasing interest. However, the precise pathophysiological role of enteric neurons in food allergies has not been elucidated. We report the presence of functional high-affinity IgE receptors (FcεRIs) in enteric neurons. FcεRI immunoreactivities were observed in approximately 70% of cholinergic myenteric neurons from choline acetyltransferase-eGFP mice. Furthermore, stimulation by IgE-antigen elevated intracellular Ca2+ concentration in isolated myenteric neurons from normal mice, suggesting that FcεRIs are capable of activating myenteric neurons. Additionally, the morphological investigation revealed that the majority of mucosal mast cells were in close proximity to enteric nerve fibers in the colonic mucosa of food allergy mice. Next, using a newly developed co-culture system of isolated myenteric neurons and mucosal-type bone-marrow-derived mast cells (mBMMCs) with a calcium imaging system, we demonstrated that the stimulation of isolated myen-teric neurons by veratridine caused the activation of mBMMCs, which was suppressed by the aden-osine A3 receptor antagonist MRE 3008F20. Moreover, the expression of the adenosine A3 receptor gene was detected in mBMMCs. Therefore, in conclusion, it is suggested that, through interaction with mucosal mast cells, IgE-antigen-activated myenteric neurons play a pathological role in further exacerbating the pathology of food allergy.

本文言語英語
論文番号1586
ジャーナルCells
10
7
DOI
出版ステータス出版済み - 2021/07

ASJC Scopus 主題領域

  • 生化学、遺伝学、分子生物学一般

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