Molecular characterization of an oxidosqualene cyclase that yields shionone, a unique tetracyclic triterpene ketone of Aster tataricus

Satoru Sawai, Hiroshi Uchiyama, Syuhei Mizuno, Toshio Aoki, Tomoyoshi Akashi, Shin Ichi Ayabe*, Takeyoshi Takahashi

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

31 被引用数 (Scopus)

抄録

Shionone is the major triterpenoid component of Aster tataricus possessing a unique all six-membered tetracyclic skeleton and 3-oxo-4-monomethyl structure. To clarify its biosynthetic process, an oxidosqualene cyclase cDNA was isolated from A. tataricus, and the function of the enzyme was determined in lanosterol synthase-deficient yeast. The cyclase yielded ca. 90% shionone and small amounts of β-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone and was designated as a shionone synthase (SHS). Transcripts of SHS were detected in A. tataricus organs, confirming its involvement in shionone biosynthesis. SHS was shown to have evolved in the Asteraceae from β-amyrin synthase lineages and acquired characteristic species- and product-specificities.

本文言語英語
ページ(範囲)1031-1036
ページ数6
ジャーナルFEBS Letters
585
7
DOI
出版ステータス出版済み - 2011/04/06

ASJC Scopus 主題領域

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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