抄録
Shionone is the major triterpenoid component of Aster tataricus possessing a unique all six-membered tetracyclic skeleton and 3-oxo-4-monomethyl structure. To clarify its biosynthetic process, an oxidosqualene cyclase cDNA was isolated from A. tataricus, and the function of the enzyme was determined in lanosterol synthase-deficient yeast. The cyclase yielded ca. 90% shionone and small amounts of β-amyrin, friedelin, dammara-20,24-dienol, and 4-epishionone and was designated as a shionone synthase (SHS). Transcripts of SHS were detected in A. tataricus organs, confirming its involvement in shionone biosynthesis. SHS was shown to have evolved in the Asteraceae from β-amyrin synthase lineages and acquired characteristic species- and product-specificities.
本文言語 | 英語 |
---|---|
ページ(範囲) | 1031-1036 |
ページ数 | 6 |
ジャーナル | FEBS Letters |
巻 | 585 |
号 | 7 |
DOI | |
出版ステータス | 出版済み - 2011/04/06 |
ASJC Scopus 主題領域
- 生物理学
- 構造生物学
- 生化学
- 分子生物学
- 遺伝学
- 細胞生物学