抄録
A microbial sensing system was developed utilizing recombinant DNA technology for the determination of environmental pollutants. The emission of light in Escherichia coli was achieved by cloning the genes encoding luciferase from firefly and by injection of the luminescence substrate luciferin. A good correlation was observed between increased luminescence and the concentration of luciferin. Measurement of environmental pollutants was based on the decrease of in vivo luminescence intensity emitted by recombinant E. coli, which was affected by cell metabolic inactivator. Environmental pollutants such as sodium azide and fluoroacetic acid, which are components of ATP-inhibiting pesticides, and antibiotics were detected at or below the μg ml-1 level by this system by measurement of the decrease in in vivo luminescence.
| 本文言語 | 英語 |
|---|---|
| ページ（範囲） | 201-206 |
| ページ数 | 6 |
| ジャーナル | Analytica Chimica Acta |
| 巻 | 244 |
| 号 | C |
| DOI | |
| 出版ステータス | 出版済み - 1991 |
ASJC Scopus 主題領域
- 分析化学
- 生化学
- 環境化学
- 分光学