Metabolomic profiling of Saposhnikoviae Radix from Mongolia by LC–IT–TOF–MS/MS and multivariate statistical analysis

Zolboo Batsukh, Kazufumi Toume, Batkhuu Javzan, Kohei Kazuma, Shao Qing Cai, Shigeki Hayashi, Nobuo Kawahara, Takuro Maruyama, Katsuko Komatsu*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

15 被引用数 (Scopus)

抄録

Saposhnikoviae Radix (SR) is a commonly used crude drug that is obtained from the root and rhizome of Saposhnikovia divaricata which is distributed throughout China, Korea, Mongolia, and Russia. To evaluate the quality of Mongolian S. divaricata, metabolomic profiling of 43 plant specimens from different regions of Mongolia, as well as 8 SR samples and 2 plant specimens from China, were conducted by liquid chromatography–ion-trap–time-of-flight–mass spectrometer (LC–IT–TOF–MS). LC–MS profiles of the specimens showed uniformity and 30 compounds were tentatively identified, including 13 chromones and 17 coumarins. Among them, 16 compounds were isolated and unambiguously verified by comparing them with the spectroscopic data of standard compounds. Orthogonal partial least squares–discriminant analysis (OPLS–DA) based on LC–MS data from 7 Mongolian specimens and 8 Chinese SR samples as well as 2 plant specimens revealed that these 2 groups were clearly distinguishable and that Mongolian specimens were characterized by an abundance of prim-O-glucosylcimifugin (1). Moreover, the OPLS–DA of the Mongolian specimens showed that they can be discriminated by their growing regions based on the content of 8 chromones. The total content of dihydrofurochromones 1–3 was relatively higher in the specimens from Khalkhgol in the far eastern part of Mongolia, while contents of 10, 11, 15, and 16 were higher in those from Holonbuir in the eastern part. Based on this research, the roots of S. divaricata from Mongolia have potential as a new resource of SR in Kampo medicine.

本文言語英語
ページ(範囲)170-188
ページ数19
ジャーナルNatural Medicines
74
1
DOI
出版ステータス出版済み - 2020/01/01

ASJC Scopus 主題領域

  • 創薬
  • 補完代替医療
  • 薬科学
  • 有機化学

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