TY - GEN
T1 - Measurement of Protein Conformational Fluctuation in Ice by Passive Millimeter-Wave Microscopy
AU - Kishigami, Akio
AU - Nozokido, Tatsuo
N1 - Publisher Copyright:
© 2018 IEEE.
PY - 2018/10/25
Y1 - 2018/10/25
N2 - Thermal radiation emitted from an aqueous solution of bovine serum albumin (BSA) was measured and analyzed by passive millimeter-wave microscopy at a temperature range low enough to freeze water and to trap conformational intermediates in BSA. Experiments were performed at a millimeter-wave frequency of 50 GHz in a temperature range from 130 K to 270 K for a 20 % BSA aqueous solution, showing a displacement between two conformational states of BSA at a boundary temperature of approximately 190 K. Our microscope system using this freeze-trapping method is expected to provide noninvasive thermal images to enable novel high-throughput calorimetry useful for the analysis of protein functions.
AB - Thermal radiation emitted from an aqueous solution of bovine serum albumin (BSA) was measured and analyzed by passive millimeter-wave microscopy at a temperature range low enough to freeze water and to trap conformational intermediates in BSA. Experiments were performed at a millimeter-wave frequency of 50 GHz in a temperature range from 130 K to 270 K for a 20 % BSA aqueous solution, showing a displacement between two conformational states of BSA at a boundary temperature of approximately 190 K. Our microscope system using this freeze-trapping method is expected to provide noninvasive thermal images to enable novel high-throughput calorimetry useful for the analysis of protein functions.
UR - http://www.scopus.com/inward/record.url?scp=85056905060&partnerID=8YFLogxK
U2 - 10.1109/IRMMW-THz.2018.8509927
DO - 10.1109/IRMMW-THz.2018.8509927
M3 - 会議への寄与
AN - SCOPUS:85056905060
T3 - International Conference on Infrared, Millimeter, and Terahertz Waves, IRMMW-THz
BT - 2018 43rd International Conference on Infrared Millimeter and Terahertz Waves, IRMMW-THz 2018
PB - IEEE Computer Society
T2 - 43rd International Conference on Infrared Millimeter and Terahertz Waves, IRMMW-THz 2018
Y2 - 9 September 2018 through 14 September 2018
ER -