Involvement of serine protease and proteinase-activated receptor 2 in dermatophyte-associated itch in mice

Tsugunobu Andoh, Yusuke Takayama, Takako Yamakoshi, Jung Bum Lee, Ayako Sano, Tadamichi Shimizu, Yasushi Kuraishi*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

22 被引用数 (Scopus)

抄録

We investigated the involvement of serine protease and proteinase-activated receptor 2 (PAR2) in dermatophyte-induced itch in mice. An intradermal injection of an extract of the dermatophyte Arthroderma vanbreuseghemii (ADV) induced hind-paw scratching, an itch-related behavior. ADV extract-induced scratching was inhibited by the opioid receptor antagonists naloxone and naltrexone, the serine protease inhibitor nafamostat mesylate, and the PAR2 receptor antagonist FSLLRY-NH2. ADV extract-induced scratching was not inhibited by the H1 histamine receptor antagonist terfenadine or by mast cell deficiency. Heat pretreatment of the ADV extract markedly reduced the scratch-inducing and serine protease activities. Proteolytic cleavage within the extracellular N terminus of the PAR2 receptor exposes a sequence that serves as a tethered ligand for the receptor. The ADV extract as well as tryptase and trypsin cleaved a synthetic N-terminal peptide of the PAR2 receptor. The present results suggest that serine protease secreted by dermatophytes causes itching through activation of the PAR2 receptors, which may be a causal mechanism of dernatophytosis itch.

本文言語英語
ページ(範囲)91-96
ページ数6
ジャーナルJournal of Pharmacology and Experimental Therapeutics
343
1
DOI
出版ステータス出版済み - 2012/10

ASJC Scopus 主題領域

  • 分子医療
  • 薬理学

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