TY - JOUR
T1 - Insulin resistance by unprocessed insulin proreceptors point mutation at the cleavage site
AU - Kobayashi, Masashi
AU - Sasaoka, Toshiyasu
AU - Takata, Yasumitsu
AU - Ishibashi, Osamu
AU - Sugibayashi, Masaaki
AU - Shigeta, Yukio
AU - Hisatomi, Akitaka
AU - Nakamura, Etsuo
AU - Tamaki, Mikio
AU - Teraoka, Hiroshi
N1 - Funding Information:
We would like to thank Drs. M.Shin for synthesizing the oligonucleotides, and O.Ohara for helpful discussions. This study was supported in part by a research grant-in-aid from the Ministry of Education, Science and Culture, and a research grant for intractable disease from the Ministry of Health and Welfare of Japan.
PY - 1988/6/16
Y1 - 1988/6/16
N2 - Failure to cleave the interconnecting site between α- and β-subunit produced insulin proreceptors in the plasma membranes which had markedly low affinity to insulin, leading to extreme insulin resistance in a patient. We performed cDNA sequence analysis of the cleavage site of the insulin proreceptor from the patient. Polymerase chain reaction was used to obtain large amount of cDNA coding for the region including the interconnecting site. A thermostable DNA polymerase, Taq polymerase, successfully produced enough amount of cDNA of the region to be sequenced. The results showed AGG (Arg) to AGT (Ser) point mutation, resulting in the change of interconnecting sequence of the two subunits from -Arg-Lys-Arg-Arg- to -Arg-Lys-Arg-Ser-. These results suggest that the tertial structure change of the cleavage site leads to production of unprocessed insulin proreceptors.
AB - Failure to cleave the interconnecting site between α- and β-subunit produced insulin proreceptors in the plasma membranes which had markedly low affinity to insulin, leading to extreme insulin resistance in a patient. We performed cDNA sequence analysis of the cleavage site of the insulin proreceptor from the patient. Polymerase chain reaction was used to obtain large amount of cDNA coding for the region including the interconnecting site. A thermostable DNA polymerase, Taq polymerase, successfully produced enough amount of cDNA of the region to be sequenced. The results showed AGG (Arg) to AGT (Ser) point mutation, resulting in the change of interconnecting sequence of the two subunits from -Arg-Lys-Arg-Arg- to -Arg-Lys-Arg-Ser-. These results suggest that the tertial structure change of the cleavage site leads to production of unprocessed insulin proreceptors.
UR - http://www.scopus.com/inward/record.url?scp=0024289963&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(88)81145-8
DO - 10.1016/S0006-291X(88)81145-8
M3 - 学術論文
C2 - 3289535
AN - SCOPUS:0024289963
SN - 0006-291X
VL - 153
SP - 657
EP - 663
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -