Insulin-degrading enzyme exists inside of rat liver peroxisomes and degrades oxidized proteins

Masashi Morita; Igor V. Kurochkin; Kiyoto Motojima; Sataro Goto; Tatsuya Takano; Shoji Okamura; Ryuichiro Sato; Sadaki Yokota; Tsuneo Imanaka

doi:10.1247/csf.25.309

Insulin-degrading enzyme exists inside of rat liver peroxisomes and degrades oxidized proteins

Masashi Morita, Igor V. Kurochkin, Kiyoto Motojima, Sataro Goto, Tatsuya Takano, Shoji Okamura, Ryuichiro Sato, Sadaki Yokota, Tsuneo Imanaka^*

^*この論文の責任著者

分子細胞機能学研究室

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

64 被引用数 (Scopus)

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Insulin-degrading enzyme (IDE) was detected by immunoblot analysis in highly purified rat liver peroxisomes. IDE in the peroxisomal fraction was resistant to proteolysis by trypsin and chymotrypsin under conditions where the peroxisomal membranes remained intact. After sonication of the peroxisomal fraction, IDE was recovered in the supernatant fraction. Further, the localization of IDE in the peroxisomes was shown by immunoelectron microscopy. In addition, IDE isolated from peroxisomes degraded insulin as well as oxidized lysozyme as a model substrate for oxidized proteins. These results suggest that IDE exists in an active form in the matrix of rat liver peroxisomes and is involved in elimination of oxidized proteins in peroxisomes.

本文言語	英語
ページ（範囲）	309-315
ページ数	7
ジャーナル	Cell Structure and Function
巻	25
号	5
DOI	https://doi.org/10.1247/csf.25.309
出版ステータス	出版済み - 2000

ASJC Scopus 主題領域

生理学
分子生物学
細胞生物学

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10.1247/csf.25.309

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title = "Insulin-degrading enzyme exists inside of rat liver peroxisomes and degrades oxidized proteins",

abstract = "Insulin-degrading enzyme (IDE) was detected by immunoblot analysis in highly purified rat liver peroxisomes. IDE in the peroxisomal fraction was resistant to proteolysis by trypsin and chymotrypsin under conditions where the peroxisomal membranes remained intact. After sonication of the peroxisomal fraction, IDE was recovered in the supernatant fraction. Further, the localization of IDE in the peroxisomes was shown by immunoelectron microscopy. In addition, IDE isolated from peroxisomes degraded insulin as well as oxidized lysozyme as a model substrate for oxidized proteins. These results suggest that IDE exists in an active form in the matrix of rat liver peroxisomes and is involved in elimination of oxidized proteins in peroxisomes.",

keywords = "Insulin-degrading enzyme, Oxidized proteins, Peroxisomes, Proteolysis, Subcellular localization",

author = "Masashi Morita and Kurochkin, {Igor V.} and Kiyoto Motojima and Sataro Goto and Tatsuya Takano and Shoji Okamura and Ryuichiro Sato and Sadaki Yokota and Tsuneo Imanaka",

year = "2000",

doi = "10.1247/csf.25.309",

language = "英語",

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journal = "Cell Structure and Function",

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publisher = "Japan Society for Cell Biology",

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TY - JOUR

T1 - Insulin-degrading enzyme exists inside of rat liver peroxisomes and degrades oxidized proteins

AU - Morita, Masashi

AU - Kurochkin, Igor V.

AU - Motojima, Kiyoto

AU - Goto, Sataro

AU - Takano, Tatsuya

AU - Okamura, Shoji

AU - Sato, Ryuichiro

AU - Yokota, Sadaki

AU - Imanaka, Tsuneo

PY - 2000

Y1 - 2000

N2 - Insulin-degrading enzyme (IDE) was detected by immunoblot analysis in highly purified rat liver peroxisomes. IDE in the peroxisomal fraction was resistant to proteolysis by trypsin and chymotrypsin under conditions where the peroxisomal membranes remained intact. After sonication of the peroxisomal fraction, IDE was recovered in the supernatant fraction. Further, the localization of IDE in the peroxisomes was shown by immunoelectron microscopy. In addition, IDE isolated from peroxisomes degraded insulin as well as oxidized lysozyme as a model substrate for oxidized proteins. These results suggest that IDE exists in an active form in the matrix of rat liver peroxisomes and is involved in elimination of oxidized proteins in peroxisomes.

AB - Insulin-degrading enzyme (IDE) was detected by immunoblot analysis in highly purified rat liver peroxisomes. IDE in the peroxisomal fraction was resistant to proteolysis by trypsin and chymotrypsin under conditions where the peroxisomal membranes remained intact. After sonication of the peroxisomal fraction, IDE was recovered in the supernatant fraction. Further, the localization of IDE in the peroxisomes was shown by immunoelectron microscopy. In addition, IDE isolated from peroxisomes degraded insulin as well as oxidized lysozyme as a model substrate for oxidized proteins. These results suggest that IDE exists in an active form in the matrix of rat liver peroxisomes and is involved in elimination of oxidized proteins in peroxisomes.

KW - Insulin-degrading enzyme

KW - Oxidized proteins

KW - Peroxisomes

KW - Proteolysis

KW - Subcellular localization

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U2 - 10.1247/csf.25.309

DO - 10.1247/csf.25.309

M3 - 学術論文

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SN - 0386-7196

VL - 25

SP - 309

EP - 315

JO - Cell Structure and Function

JF - Cell Structure and Function

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Insulin-degrading enzyme exists inside of rat liver peroxisomes and degrades oxidized proteins

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