Influence of injection timing on severity of cadmium-induced testicular toxicity in mice

Katsumi Ohtani, Yukie Yanagiba, Atsushige Ashimori, Asuka Takeuchi, Naoko Takada, Masako Togawa, Tatsuya Hasegawa, Masayuki Ikeda, Nobuhiko Miura*

*この論文の責任著者

研究成果: ジャーナルへの寄稿Letter査読

18 被引用数 (Scopus)

抄録

Cadmium (Cd) is one of the endocrine disrupter and is a well-known testicular toxicant. Recently, we reported that Cd-induced mortality was markedly different by injection timing. In this report, we investigated whether severity of testicular toxicity was affected by injection timing of Cd. C57BL/6J mice (male, 7 w) were received single intraperitoneal injection of CdCl2 (4.5 mg/kg) at zeitgeber time 6 (ZT6) or ZT18; these injection timings showed highest (ZT6) or lowest (ZT18) mortality in our previous study (Miura, 2012). After one week of the injection, several parameters for testicular toxicity such as epididymal sperm motility and numbers of sperm head both in cauda epididymidis and testis were measured. At ZT6 injection group, all parameters examined were significantly reduced compared to the control group. However, very interestingly, no significant changes were observed at ZT18 injection group. We obtained similar results by another experiment in which mice were received single subcutaneous injection of CdCl2 (4 or 6 mg/kg) followed by measuring the parameters ten days after the injection. This diurnal variation was not contradictory to the result of the lethal toxicity which we showed earlier. Therefore, our results indicate that the testicular toxicity of Cd is also influenced by the injection timing.

本文言語英語
ページ(範囲)145-150
ページ数6
ジャーナルJournal of Toxicological Sciences
38
1
DOI
出版ステータス出版済み - 2013/02

ASJC Scopus 主題領域

  • 毒物学

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