Induction of regulatory T Cells as a novel mechanism underlying the therapeutic action of Kakkonto, a traditional Japanese herbal medicine, in a murine food allergy model

Takeshi Yamamoto*, Kanae Fujiwara, Yuma Tsubota, Natsuko Kageyama-Yahara, Shusaku Hayashi, Makoto Kadowaki

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

21 被引用数 (Scopus)

抄録

Background: The number of patients with food allergy (FA) has dramatically increased. Although satisfactory drug therapies for FA are not available, we have found that kakkonto, a traditional Japanese herbal medicine, suppressed the occurrence of allergic symptoms in an FA mouse model. Thus, we investigated whether kakkonto could regulate the activation and differentiation of T cells in the colon. Methods: BALB/c mice were systemically sensitized and then orally challenged with ovalbumin. FA mice were orally treated with kakkonto. Lamina propria (LP) cells from their colons were isolated and analyzed. Results: Kakkonto significantly reduced the proportion of CD69+ cells and the elevated helper T cell type 2-specific transcription factor GATA-3 mRNA expression in the LP CD4+ T cells, showing that kakkonto has a suppressive effect on the activation and Th2 differentiation of LP effector CD4+ T cells of the FA mouse colon. Furthermore, kakkonto significantly increased the proportion of Foxp3+CD4+ regulatory T cells in the LP CD4+ T cells of the FA mouse colon. Similarly, the number of Foxp3-positive cells was dramatically increased in the colonic mucosa of kakkonto-administered FA mice. However, the pharmacological effect and Foxp3+CD4+ regulatory T cell-inducing ability of kakkonto were not attenuated by the administration of an anti-CD25 monoclonal antibody in the FA model. Conclusions: The induction of Foxp3+CD4+CD25- regulatory T cells in the colon as a novel mechanism underlying the therapeutic action of kakkonto could be utilized for the development of a novel anti-FA drug.

本文言語英語
ページ(範囲)146-156
ページ数11
ジャーナルInternational Archives of Allergy and Immunology
169
3
DOI
出版ステータス出版済み - 2016/06/01

ASJC Scopus 主題領域

  • 免疫アレルギー学
  • 免疫学

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