TY - CONF
T1 - Increased behavioral activity with regular circadian rhythm in PACAP specific receptor (PAC1) transgenic mice
AU - Arata, Satoru
AU - Hosono, Tomohiko
AU - Taketomi, Yoshitaka
AU - Kageyama, Haruaki
AU - Nakamachi, Tomoya
AU - Shioda, Seiji
PY - 2009
Y1 - 2009
N2 - To define the physiological roles of Pituitary adenylate cyclase-activating polypeptide (PACAP), we developed PACAP specific receptor (PAC1) transgenic (Tg) mice using the Cre/loxP recombination system. wPAC1 Tg mice, which express the Pac1 transgene in their entire body, showed increased behavioral activity in the dark period of a 12-h light/dark cycle (Zeitegeber time 0 (ZT0) = lights on, ZT12= lights off). LNL-PAC1/CaMKII-Cre double Tg mice (hPAC1 Tg mice), which undergo Cre/loxP recombination in hippocampal nerve cells, expressed the Pac1 transgene in the hippocampus. hPAC1 Tg mice showed increased behavioral activity (150% or more compared to control) in the early dark period (ZT 12-17), which is the most active time for wild type mice. However, there was no change in circadian rhythm. Similar hyperactivity was observed in LNL-PAC1/CaMKII-CreER double Tg mice (ihPAC1 Tg mice) when the mice were treated with tamoxifen to induce Cre/loxp recombination in adulthood. Moreover, immunohistochemical analysis of LNL-PAC1/LNL-Venus/ CaMKII-CreER triple Tg mice treated with tamoxifen, revealed that PAC1 transgene were expressed in hippocampal nerve cells. These results suggest that the expression of the PAC1 transgene, especially in hippocampal nerve cells, may enhance the behavioral activity associated with regular circadian rhythm.
AB - To define the physiological roles of Pituitary adenylate cyclase-activating polypeptide (PACAP), we developed PACAP specific receptor (PAC1) transgenic (Tg) mice using the Cre/loxP recombination system. wPAC1 Tg mice, which express the Pac1 transgene in their entire body, showed increased behavioral activity in the dark period of a 12-h light/dark cycle (Zeitegeber time 0 (ZT0) = lights on, ZT12= lights off). LNL-PAC1/CaMKII-Cre double Tg mice (hPAC1 Tg mice), which undergo Cre/loxP recombination in hippocampal nerve cells, expressed the Pac1 transgene in the hippocampus. hPAC1 Tg mice showed increased behavioral activity (150% or more compared to control) in the early dark period (ZT 12-17), which is the most active time for wild type mice. However, there was no change in circadian rhythm. Similar hyperactivity was observed in LNL-PAC1/CaMKII-CreER double Tg mice (ihPAC1 Tg mice) when the mice were treated with tamoxifen to induce Cre/loxp recombination in adulthood. Moreover, immunohistochemical analysis of LNL-PAC1/LNL-Venus/ CaMKII-CreER triple Tg mice treated with tamoxifen, revealed that PAC1 transgene were expressed in hippocampal nerve cells. These results suggest that the expression of the PAC1 transgene, especially in hippocampal nerve cells, may enhance the behavioral activity associated with regular circadian rhythm.
M3 - 学会論文
SP - 199-
ER -