Increased 5′-nucleotidase activity induced by dibutyryl cyclic AMP treatment of cultured glial cells

Shigeru Amano*, Fumitada Hazama, Jun Kawai, Masakiyo Sasahara

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

18 被引用数 (Scopus)

抄録

In an attempt to clarify the relationship between ecto-5′-nucleotidase (5′-N) activity and cell differentiation of glial cells, dibutyryl cyclic AMP (dBcAMP), which induces cell differentiation, was administrated to cultured rat glioma cells in logarithmic and confluent phases of cultivation. To evaluate the cellular differentiation, cell morphology and the number of glial fibrillary acidic protein (GFAP) positive cells were examined. Treatment with 1 mM dBcAMP decreased cell proliferation and induced cell differentiation in both the logarithmic and the confluent phases. The number of GFAP-positive cells increased with cellular aging and this tendency was enhanced by dBcAMP administration. Ecto-5′-N activity was higher in dBcAMP treated cells than in non-treated cells in both the logarithmic and the confluent phases. These findings suggest that ecto-5′-N activity of C6 glioma cells is increased by dBcAMP actions and is accompanied by cell differentiation.

本文言語英語
ページ(範囲)254-258
ページ数5
ジャーナルBrain Research
506
2
DOI
出版ステータス出版済み - 1990/01/08

ASJC Scopus 主題領域

  • 神経科学一般
  • 分子生物学
  • 臨床神経学
  • 発生生物学

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