Impact of aging and life-long calorie restriction on expression of apoptosis-related genes in male F344 rat liver

Kohei Ando, Yoshikazu Higami*, Tomoshi Tsuchiya, Takashi Kanematsu, Isao Shimokawa

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

37 被引用数 (Scopus)

抄録

Aging enhances apoptosis of hepatocytes under normal physiological conditions and increases the susceptibility to apoptosis of hepatocytes, whereas chronic calorie restriction (CR) suppresses the age-enhanced susceptibility to apoptosis. To clarify the subcellular mechanisms of age-associated dysregulation of apoptosis and the effects of CR, we analyzed the expression of genes promoting apoptosis (p53, Fas receptor, Fas ligand, TNF receptor 1, TNFα, Bax, TGFβ1) and genes preventing apoptosis (Bcl-2 and Bcl-XL) in the livers of 3-, 6-, 15-, and 24-month-old male F344 rats that were either fed ad libitum or subjected to a 30% reduction in food intake (CR). After the age of 6 months, expression of p53, Fas receptor, Fas ligand, and TNFα mRNAs was up-regulated with aging. CR suppressed this age-enhanced p53 and Fas receptor mRNA expression, but expression of the other genes was not altered significantly by aging or CR. Expression of Fas receptor in hepatocytes, as detected immunohistochemically, increased with age, but CR suppressed ageaccelerated Fas receptor expression. Our findings suggest that TNF ligand/TNF receptor family signaling, particularly Fas receptor expression, is important in age- and CR-modulated apoptosis of hepatocytes. Hepatocytes that were immunoreactive for p53 had slightly increased with aging, suggesting that p53 may mediate the age-enhanced up-regulation of Fas receptor in hepatocytes.

本文言語英語
ページ(範囲)293-300
ページ数8
ジャーナルMicroscopy Research and Technique
59
4
DOI
出版ステータス出版済み - 2002/11/15

ASJC Scopus 主題領域

  • 解剖学
  • 組織学
  • 器械工学
  • 医療検査技術

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