@article{5edb5d00977e4c17af94c27ce374b88b,
title = "Immunohistochemical localization of neuronal nicotinic receptor subtypes at the pre- and postjunctional sites in mouse diaphragm muscle",
abstract = "The existence of neuronal nicotinic acetylcholine receptor (nAChR) subunits was investigated in the cryostat sections of mouse diaphragm muscles using the indirect immunofluorescence technique. The specific immunolabelings with monoclonal antibodies (mAbs) to β2 and to α8 subunits of neuronal nAChR were observed at the endplate determined by labeling with a fluorescent dye (BODIPY)-conjugated α-bungarotoxin. The immunoreactivity of mAb to the α3 subunit of neuronal nAChR was detected on the motor nerve fibers including the nerve terminals. These results provide evidence that the subtypes of postsynaptic nAChR, recognized by the anti-β2 and/or anti-α8 mAbs, and the presynaptic nAChR recognized by the anti-α3 mAb, are present at the neuromuscular junction, in addition to the classical muscle nAChR.",
keywords = "Immunohistochemistry, Monoclonal antibody, Motor nerve, Neuromuscular junction, Neuronal nicotinic acetylcholine receptor, Skeletal muscle",
author = "Hiroshi Tsuneki and Ikuko Kimura and Katsuya Dezaki and Masayasu Kimura and Carlo Sala and Guido Fumagalli",
note = "Funding Information: We thank Professor J. Lindstrom (University of Pennsylvania) and Professor D.K. Berg (University of California, San Diego) for providing all the mAbs used and for their helpful comments, and Mr. S. Yanagi for his technical assistance. This work was supported by Grant-in-Aid for JSPS Fellow,; (No. 061200 to H.T.) from the Ministry of Education, Science and Culture, Japan.",
year = "1995",
month = aug,
day = "18",
doi = "10.1016/0304-3940(95)11824-G",
language = "英語",
volume = "196",
pages = "13--16",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",
}