Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localization

Tadamichi Shimizu; Akira Ohkawara; Jun Nishihira; Wataru Sakamoto

doi:10.1016/0014-5793(96)00120-2

Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localization

Tadamichi Shimizu, Akira Ohkawara, Jun Nishihira^*, Wataru Sakamoto

^*この論文の責任著者

皮膚科学講座

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

139 被引用数 (Scopus)

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The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin mere examined. Reverse transcription-polymerase chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further confirmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyclonal antibody against human recombinant MIF. Immunohistochemical studies showed that MIF existed in human epidermis, especially in the basal layer. The pathophysiological role of MIF in human skin remains undefined; however, the present results indicate that MIF may play an important role in immunity, inflammation and cellular differentiation of epidermal cells.

本文言語	英語
ページ（範囲）	199-202
ページ数	4
ジャーナル	FEBS Letters
巻	381
号	3
DOI	https://doi.org/10.1016/0014-5793(96)00120-2
出版ステータス	出版済み - 1996/03/04

ASJC Scopus 主題領域

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/0014-5793(96)00120-2

引用スタイル

@article{57e5386f25f54aaba471ee60e980fe5a,

title = "Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localization",

abstract = "The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin mere examined. Reverse transcription-polymerase chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further confirmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyclonal antibody against human recombinant MIF. Immunohistochemical studies showed that MIF existed in human epidermis, especially in the basal layer. The pathophysiological role of MIF in human skin remains undefined; however, the present results indicate that MIF may play an important role in immunity, inflammation and cellular differentiation of epidermal cells.",

keywords = "Human skin, Immunohistochemistry, Keratinocyte, Macrophage migration inhibitory factor, Reverse transcription-polymerase chain reaction",

author = "Tadamichi Shimizu and Akira Ohkawara and Jun Nishihira and Wataru Sakamoto",

note = "Funding Information: AcknowledgemenTths:is researchw as supportedb y a Grant-in-Aid for Research(n o. 07670162f)r omthe Ministryo f EducationS, cience and Cultureo f Japan, The AkiyamaF oundationa, nd The Ohyama FoundationW. e are gratefutlo T. KuriyamaS . Tone for his technical assistance.",

year = "1996",

month = mar,

day = "4",

doi = "10.1016/0014-5793(96)00120-2",

language = "英語",

volume = "381",

pages = "199--202",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localization

AU - Shimizu, Tadamichi

AU - Ohkawara, Akira

AU - Nishihira, Jun

AU - Sakamoto, Wataru

N1 - Funding Information: AcknowledgemenTths:is researchw as supportedb y a Grant-in-Aid for Research(n o. 07670162f)r omthe Ministryo f EducationS, cience and Cultureo f Japan, The AkiyamaF oundationa, nd The Ohyama FoundationW. e are gratefutlo T. KuriyamaS . Tone for his technical assistance.

PY - 1996/3/4

Y1 - 1996/3/4

N2 - The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin mere examined. Reverse transcription-polymerase chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further confirmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyclonal antibody against human recombinant MIF. Immunohistochemical studies showed that MIF existed in human epidermis, especially in the basal layer. The pathophysiological role of MIF in human skin remains undefined; however, the present results indicate that MIF may play an important role in immunity, inflammation and cellular differentiation of epidermal cells.

AB - The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin mere examined. Reverse transcription-polymerase chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further confirmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyclonal antibody against human recombinant MIF. Immunohistochemical studies showed that MIF existed in human epidermis, especially in the basal layer. The pathophysiological role of MIF in human skin remains undefined; however, the present results indicate that MIF may play an important role in immunity, inflammation and cellular differentiation of epidermal cells.

KW - Human skin

KW - Immunohistochemistry

KW - Keratinocyte

KW - Macrophage migration inhibitory factor

KW - Reverse transcription-polymerase chain reaction

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U2 - 10.1016/0014-5793(96)00120-2

DO - 10.1016/0014-5793(96)00120-2

M3 - 学術論文

C2 - 8601455

AN - SCOPUS:0029985302

SN - 0014-5793

VL - 381

SP - 199

EP - 202

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Identification of macrophage migration inhibitory factor (MIF) in human skin and its immunohistochemical localization

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