Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus

Jing Chen; Hiroyuki Morita; Ryohei Kato; Hiroshi Noguchi; Shigetoshi Sugio; Ikuro Abe

doi:10.1107/S1744309112004964

Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus

Jing Chen, Hiroyuki Morita, Ryohei Kato, Hiroshi Noguchi, Shigetoshi Sugio^*, Ikuro Abe

^*この論文の責任著者

和漢医薬学総合研究所研究開発部門資源開発分野（天然物創薬学領域）

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

4 被引用数 (Scopus)

抄録

CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to space group P2 ₁2 ₁2 ₁, with unit-cell parameters a = 84.4, b = 157.1, c = 161.8 Å, α = β = γ = 90.0°.

本文言語	英語
ページ（範囲）	355-358
ページ数	4
ジャーナル	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
巻	68
号	3
DOI	https://doi.org/10.1107/S1744309112004964
出版ステータス	出版済み - 2012/03

ASJC Scopus 主題領域

生物理学
構造生物学
生化学
遺伝学
凝縮系物理学

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10.1107/S1744309112004964

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title = "Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus",

abstract = "CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to space group P2 12 12 1, with unit-cell parameters a = 84.4, b = 157.1, c = 161.8 {\AA}, α = β = γ = 90.0°.",

keywords = "alkaloids, prenyltransferases, tryptophan",

author = "Jing Chen and Hiroyuki Morita and Ryohei Kato and Hiroshi Noguchi and Shigetoshi Sugio and Ikuro Abe",

year = "2012",

month = mar,

doi = "10.1107/S1744309112004964",

language = "英語",

volume = "68",

pages = "355--358",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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T1 - Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus

AU - Chen, Jing

AU - Morita, Hiroyuki

AU - Kato, Ryohei

AU - Noguchi, Hiroshi

AU - Sugio, Shigetoshi

AU - Abe, Ikuro

PY - 2012/3

Y1 - 2012/3

N2 - CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to space group P2 12 12 1, with unit-cell parameters a = 84.4, b = 157.1, c = 161.8 Å, α = β = γ = 90.0°.

AB - CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to space group P2 12 12 1, with unit-cell parameters a = 84.4, b = 157.1, c = 161.8 Å, α = β = γ = 90.0°.

KW - alkaloids

KW - prenyltransferases

KW - tryptophan

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DO - 10.1107/S1744309112004964

M3 - 学術論文

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AN - SCOPUS:84858966622

SN - 1744-3091

VL - 68

SP - 355

EP - 358

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 3

ER -

Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus

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