TY - JOUR
T1 - Expression of platelet-derived growth factor B-chain in the mature rat brain and pituitary gland
AU - Sasahara, Masakiyo
AU - Sato, Hiroshi
AU - Iihara, Koji
AU - Wang, Jian
AU - Chue, Chin Hui
AU - Takayama, Shoukei
AU - Hayase, Yoneko
AU - Hazama, Fumitada
N1 - Funding Information:
We thank Prof. Russell Ross and Prof. Richard J. Johnson, University of Washington, Seattle, USA, for their gift of the cDNA for the human PDGF B-chain. We are also grateful to Dr. K. Kurokawa from Shiga University of Medical Science for useful scientific discussions, and to Ms. S. Hirayama for excellent technical assistance. We thank Mr. P. Schneider for assistance in preparing the manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (no. 05770148, no. 06670223).
PY - 1995/8
Y1 - 1995/8
N2 - For better understanding of the role of platelet-derived growth factor (PDGF) B-chain in the brain, the expression of PDGF B-chain was studied in the mature rat brain at both protein and mRNA levels, by assay of PDGF B-chain-related mitogenic activity, Northern blot, in situ hybridization and immunohistochemistry. It was shown that (1) mature rat brain contained substantial PDGF B-chain-related mitogenic activity, (2) significant amounts of two sizes of transcripts (3.5 kb, widely, and 2.6 kb, weakly and in narrower areas) were expressed in the brain, and (3) the transcripts were localized in ubiquitous neurons by in situ hybridization, with the strongest signal in hippocampal pyramidal cells, which distribution almost corresponded with that of the immunoreactive products. The abundant neuronal localization of the transcript and protein, as well as the neuronal expression of the receptor reported elsewhere, suggests the role of the growth factor in neuronal cells as a neuronal regulatory and/or trophic agent acting by autocrine loop or by neuron to neuron interaction. However, there was an apparent discrepancy in part, in the distribution between transcripts and immunoreactivity; that is, transcripts were expressed intensely in the intermediate pituitary lobe with only a scattered immunoreactivity, and the opposite situation was observed in the accessory olfactory nerve and posterior pituitary lobe. This might suggest that PDGF B-chain is transported or secreted in these foci.
AB - For better understanding of the role of platelet-derived growth factor (PDGF) B-chain in the brain, the expression of PDGF B-chain was studied in the mature rat brain at both protein and mRNA levels, by assay of PDGF B-chain-related mitogenic activity, Northern blot, in situ hybridization and immunohistochemistry. It was shown that (1) mature rat brain contained substantial PDGF B-chain-related mitogenic activity, (2) significant amounts of two sizes of transcripts (3.5 kb, widely, and 2.6 kb, weakly and in narrower areas) were expressed in the brain, and (3) the transcripts were localized in ubiquitous neurons by in situ hybridization, with the strongest signal in hippocampal pyramidal cells, which distribution almost corresponded with that of the immunoreactive products. The abundant neuronal localization of the transcript and protein, as well as the neuronal expression of the receptor reported elsewhere, suggests the role of the growth factor in neuronal cells as a neuronal regulatory and/or trophic agent acting by autocrine loop or by neuron to neuron interaction. However, there was an apparent discrepancy in part, in the distribution between transcripts and immunoreactivity; that is, transcripts were expressed intensely in the intermediate pituitary lobe with only a scattered immunoreactivity, and the opposite situation was observed in the accessory olfactory nerve and posterior pituitary lobe. This might suggest that PDGF B-chain is transported or secreted in these foci.
KW - Mitogenic activity
KW - Neuron
KW - Pituitary gland
KW - Platelet-derived growth factor B-chain
KW - Rat brain
KW - to situ hybridization
UR - http://www.scopus.com/inward/record.url?scp=0029050669&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(95)00060-6
DO - 10.1016/0169-328X(95)00060-6
M3 - 学術論文
C2 - 7494464
AN - SCOPUS:0029050669
SN - 0169-328X
VL - 32
SP - 63
EP - 74
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -