TY - JOUR
T1 - Expression of filaggrin-2 protein in the epidermis of human skin diseases
T2 - A comparative analysis with filaggrin
AU - Makino, Teruhiko
AU - Mizawa, Megumi
AU - Yamakoshi, Takako
AU - Takaishi, Mikiro
AU - Shimizu, Tadamichi
N1 - Funding Information:
We sincerely thank Mrs. Nanase Sawada and Mrs. Kozue Urata for their excellent technical support. This work was supported by a Grant-in-aid 24591643 (to T.M.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan .
PY - 2014/6/20
Y1 - 2014/6/20
N2 - Filaggrin-2 is a member of the S100 fused-type protein family, and the structural features and expression of filaggrin-2 are similar to those of profilaggrin, a protein essential for keratinization. In the present study, we investigated the expression profile of filaggrin-2 in patients with skin diseases using antibodies against the repetitive region of filaggrin-2. In tissue samples from patients with skin diseases which are associated with a decrease in filaggrin, including ichthyosis vulgaris, atopic dermatitis and psoriasis vulgaris, the expression level of filaggrin-2 was markedly decreased compared to that in normal skin samples. In contrast, the expression of filaggrin-2 increased in parallel with that of filaggrin in samples of tissue from patients with skin diseases associated with hyperkeratosis, such as lichen planus and epidermolytic ichthyosis. Interestingly, filaggrin-2 signals were observed in slightly higher layers of the epidermis in comparison to those of filaggrin. Similarly, the expression of filaggrin-2 proteins was induced slightly later than filaggrin in the cultured keratinocytes. These findings suggest that filaggrin-2 may play an overlapping role with filaggrin in epithelial cornification; however, it may also have a partially distinct role in the molecular processes of cornification.
AB - Filaggrin-2 is a member of the S100 fused-type protein family, and the structural features and expression of filaggrin-2 are similar to those of profilaggrin, a protein essential for keratinization. In the present study, we investigated the expression profile of filaggrin-2 in patients with skin diseases using antibodies against the repetitive region of filaggrin-2. In tissue samples from patients with skin diseases which are associated with a decrease in filaggrin, including ichthyosis vulgaris, atopic dermatitis and psoriasis vulgaris, the expression level of filaggrin-2 was markedly decreased compared to that in normal skin samples. In contrast, the expression of filaggrin-2 increased in parallel with that of filaggrin in samples of tissue from patients with skin diseases associated with hyperkeratosis, such as lichen planus and epidermolytic ichthyosis. Interestingly, filaggrin-2 signals were observed in slightly higher layers of the epidermis in comparison to those of filaggrin. Similarly, the expression of filaggrin-2 proteins was induced slightly later than filaggrin in the cultured keratinocytes. These findings suggest that filaggrin-2 may play an overlapping role with filaggrin in epithelial cornification; however, it may also have a partially distinct role in the molecular processes of cornification.
KW - Atopic dermatitis
KW - Filaggrin
KW - Filaggrin-2
KW - Ichthyosis vulgaris
KW - Psoriasis vulgaris
KW - S100 fused-protein family
UR - http://www.scopus.com/inward/record.url?scp=84901756422&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2014.04.165
DO - 10.1016/j.bbrc.2014.04.165
M3 - 学術論文
C2 - 24813994
AN - SCOPUS:84901756422
SN - 0006-291X
VL - 449
SP - 100
EP - 106
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -
