Evidence of functional ryanodine receptors in rat mesenteric collecting lymphatic vessels

Michiko Jo, Andrea N. Trujillo, Ying Yang, Jerome W. Breslin*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

20 被引用数 (Scopus)

抄録

In the current study, the potential contributions of ryanodine receptors (RyRs) to intrinsic pumping and responsiveness to substance P (SP) were investigated in isolated rat mesenteric collecting lymphatic vessels. Responses to SP were characterized in lymphatic vessels in the absence or presence of pretreatment with nifedipine to block L-type Ca2+ channels, caffeine to block normal release and uptake of Ca2+ from the sarcoplasmic reticulum, ryanodine to block all RyR isoforms, or dantrolene to more selectively block RyR1 and RyR3. RyR expression and localization in lymphatics was also assessed by quantitative PCR and immunofluorescence confocal microscopy. The results show that SP normally elicits a significant increase in contraction frequency and a decrease in end-diastolic diameter. In the presence of nifedipine, phasic contractions stop, yet subsequent SP treatment still elicits a strong tonic contraction. Caffeine treatment gradually relaxes lymphatics, causing a loss of phasic contractions, and prevents subsequent SP-induced tonic contraction. Ryanodine also gradually diminishes phasic contractions but without causing vessel relaxation and significantly inhibits the SP-induced tonic contraction. Dantrolene treatment did not significantly impair lymphatic contractions nor the response to SP. The mRNA for all RyR isoforms is detectable in isolated lymphatics. RyR2 and RyR3 proteins are found predominantly in the collecting lymphatic smooth muscle layer. Collectively, the data suggest that SP-induced tonic contraction requires both extracellular Ca2+ plus Ca2+ release from internal stores and that RyRs play a role in the normal contractions and responsiveness to SP of rat mesenteric collecting lymphatics.

本文言語英語
ページ(範囲)H561-H574
ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
317
3
DOI
出版ステータス出版済み - 2019/09

ASJC Scopus 主題領域

  • 生理学
  • 循環器および心血管医学
  • 生理学(医学)

フィンガープリント

「Evidence of functional ryanodine receptors in rat mesenteric collecting lymphatic vessels」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル