TY - JOUR
T1 - Evaluation of chikusetsusaponin IVa isolated from Alternanthera philoxeroides for its potency against viral replication
AU - Rattanathongkom, Ariya
AU - Lee, Jung Bum
AU - Hayashi, Kyoko
AU - Sripanidkulchai, Bung Orn
AU - Kanchanapoom, Tripetch
AU - Hayashi, Toshimitsu
PY - 2009/6
Y1 - 2009/6
N2 - Chikusetsusaponin IVa and calenduloside E were isolated from the whole plant of Alternanthera philoxeroides (Mart.) Griseb (Amaranthaceae) and evaluated for their antiviral activities. Chikusetsusaponin IVa showed antiviral activities against HSV-1, HSV-2, human cytomegalovirus, measles virus, and mumps virus with selectivity indices (CC50/IC50) of 29, 30, 73, 25, and 25, respectively. On the other hand, calenduloside E showed no antiviral effects against any of the viruses tested. The mode of HSV-2 action of chikusetsusaponin IVa was determined under different experimental conditions. The anti-HSV-2 target of the compound might be mainly related to direct inactivation of virus particles and to the inhibition of release of progeny viruses from infected cells, but it is not related to inhibition of viral attachment, cell penetration, and viral protein synthesis. This compound also provided in vivo efficacy in a mouse model of genital herpes caused by HSV-2. These results demonstrate that chikusetsusaponin IVa might be a candidate of antiherpetic agents.
AB - Chikusetsusaponin IVa and calenduloside E were isolated from the whole plant of Alternanthera philoxeroides (Mart.) Griseb (Amaranthaceae) and evaluated for their antiviral activities. Chikusetsusaponin IVa showed antiviral activities against HSV-1, HSV-2, human cytomegalovirus, measles virus, and mumps virus with selectivity indices (CC50/IC50) of 29, 30, 73, 25, and 25, respectively. On the other hand, calenduloside E showed no antiviral effects against any of the viruses tested. The mode of HSV-2 action of chikusetsusaponin IVa was determined under different experimental conditions. The anti-HSV-2 target of the compound might be mainly related to direct inactivation of virus particles and to the inhibition of release of progeny viruses from infected cells, but it is not related to inhibition of viral attachment, cell penetration, and viral protein synthesis. This compound also provided in vivo efficacy in a mouse model of genital herpes caused by HSV-2. These results demonstrate that chikusetsusaponin IVa might be a candidate of antiherpetic agents.
KW - Alternanthera philoxeroides (Amarantheaceae)
KW - Antiviral activity
KW - Chikusetsusaponin IV a
KW - Genital herpes
KW - Herpes simplex virus type 2
UR - http://www.scopus.com/inward/record.url?scp=69049083430&partnerID=8YFLogxK
U2 - 10.1055/s-0029-1185436
DO - 10.1055/s-0029-1185436
M3 - 学術論文
C2 - 19277947
AN - SCOPUS:69049083430
SN - 0032-0943
VL - 75
SP - 829
EP - 835
JO - Planta Medica
JF - Planta Medica
IS - 8
ER -
