抄録
Two genes involved in δ-guaiene biosynthesis in Aquilaria microcarpa, δ-guaiene synthase (GS) and farnesyl diphosphate synthase (FPS), were overexpressed in Escherichia coli cells. Immunoblot analysis revealed that the concentration of GS-translated protein was rather low in the cells transformed by solely GS while appreciable accumulation of the recombinant protein was observed when GS was coexpressed with FPS. GS-transformed cells liberated only a trace amount of δ-guaiene (0.004 δg/mL culture), however, the concentration of the compound elevated to 0.08 δg/mL culture in the cells transformed by GS plus FPS. δ-Guaiene biosynthesis was markedly activated when E. coli cells coexpressing GS and FPS were incubated in enriched Terrific broth, and the content of the compound increased to approximately 0.6 δg/mL culture. These results suggest that coexpression of FPS and GS in E. coli is required for efficient δ-guaiene production in the bacterial cells, and the sesquiterpene-producing activity of the transformant is appreciably enhanced in the nutrients-enriched medium.
本文言語 | 英語 |
---|---|
ページ(範囲) | 1221-1224 |
ページ数 | 4 |
ジャーナル | Natural Product Communications |
巻 | 11 |
号 | 9 |
DOI | |
出版ステータス | 出版済み - 2016/09 |
ASJC Scopus 主題領域
- 薬理学
- 植物科学
- 創薬
- 補完代替医療