Engineered biosynthesis of plant polyketides: Manipulation of chalcone synthase

Ikuro Abe; Tatsuya Watanabe; Hiroyuki Morita; Toshiyuki Kohno; Hiroshi Noguchi

doi:10.1021/ol052912h

Engineered biosynthesis of plant polyketides: Manipulation of chalcone synthase

Ikuro Abe^*, Tatsuya Watanabe, Hiroyuki Morita, Toshiyuki Kohno, Hiroshi Noguchi

^*この論文の責任著者

和漢医薬学総合研究所研究開発部門資源開発分野（天然物創薬学領域）

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

44 被引用数 (Scopus)

抄録

Chalcone synthase (CHS) is a plant-specific type III polyketide synthase catalyzing condensation of 4-coumaroyl-CoA with three molecules of malonyl-CoA. Surprisingly, it was demonstrated that S338V mutant of Scutellaria baicalensis CHS produced octaketides SEK4/SEK4b from eight molecules of malonyl-CoA. Further, the octaketides-forming activity was dramatically increased in a CHS triple mutant (T197G/G256L/ S338T). The functional conversion is based on the simple steric modulation of a chemically inert residue lining the active-site cavity.

本文言語	英語
ページ（範囲）	499-502
ページ数	4
ジャーナル	Organic Letters
巻	8
号	3
DOI	https://doi.org/10.1021/ol052912h
出版ステータス	出版済み - 2006/02/02

ASJC Scopus 主題領域

生化学
物理化学および理論化学
有機化学

文献へのアクセス

10.1021/ol052912h

引用スタイル

@article{3f2acfd949654db28f87e1773c1469d0,

title = "Engineered biosynthesis of plant polyketides: Manipulation of chalcone synthase",

abstract = "Chalcone synthase (CHS) is a plant-specific type III polyketide synthase catalyzing condensation of 4-coumaroyl-CoA with three molecules of malonyl-CoA. Surprisingly, it was demonstrated that S338V mutant of Scutellaria baicalensis CHS produced octaketides SEK4/SEK4b from eight molecules of malonyl-CoA. Further, the octaketides-forming activity was dramatically increased in a CHS triple mutant (T197G/G256L/ S338T). The functional conversion is based on the simple steric modulation of a chemically inert residue lining the active-site cavity.",

author = "Ikuro Abe and Tatsuya Watanabe and Hiroyuki Morita and Toshiyuki Kohno and Hiroshi Noguchi",

year = "2006",

month = feb,

day = "2",

doi = "10.1021/ol052912h",

language = "英語",

volume = "8",

pages = "499--502",

journal = "Organic Letters",

issn = "1523-7060",

publisher = "American Chemical Society",

number = "3",

}

TY - JOUR

T1 - Engineered biosynthesis of plant polyketides

T2 - Manipulation of chalcone synthase

AU - Abe, Ikuro

AU - Watanabe, Tatsuya

AU - Morita, Hiroyuki

AU - Kohno, Toshiyuki

AU - Noguchi, Hiroshi

PY - 2006/2/2

Y1 - 2006/2/2

N2 - Chalcone synthase (CHS) is a plant-specific type III polyketide synthase catalyzing condensation of 4-coumaroyl-CoA with three molecules of malonyl-CoA. Surprisingly, it was demonstrated that S338V mutant of Scutellaria baicalensis CHS produced octaketides SEK4/SEK4b from eight molecules of malonyl-CoA. Further, the octaketides-forming activity was dramatically increased in a CHS triple mutant (T197G/G256L/ S338T). The functional conversion is based on the simple steric modulation of a chemically inert residue lining the active-site cavity.

AB - Chalcone synthase (CHS) is a plant-specific type III polyketide synthase catalyzing condensation of 4-coumaroyl-CoA with three molecules of malonyl-CoA. Surprisingly, it was demonstrated that S338V mutant of Scutellaria baicalensis CHS produced octaketides SEK4/SEK4b from eight molecules of malonyl-CoA. Further, the octaketides-forming activity was dramatically increased in a CHS triple mutant (T197G/G256L/ S338T). The functional conversion is based on the simple steric modulation of a chemically inert residue lining the active-site cavity.

UR - http://www.scopus.com/inward/record.url?scp=32644439876&partnerID=8YFLogxK

U2 - 10.1021/ol052912h

DO - 10.1021/ol052912h

M3 - 学術論文

C2 - 16435869

AN - SCOPUS:32644439876

SN - 1523-7060

VL - 8

SP - 499

EP - 502

JO - Organic Letters

JF - Organic Letters

IS - 3

ER -

Engineered biosynthesis of plant polyketides: Manipulation of chalcone synthase

抄録

ASJC Scopus 主題領域

文献へのアクセス

フィンガープリント

引用スタイル