Electrochemical characterization of the polyaniline-based oxidase-peroxidase electrode and application to the enzyme switch

The electrochemical properties of oxidase-peroxidase co-immobilized electrode fabricated electrochemically deposited polyaniline (PAn) were investigated. It found that electro-conductivity of a PAn film was necessary for the oxidase-HRP to show its function. Based on this result, the enzyme switch utilizing the oxidase-HRP system was developed by utilizing electro-conductivity changes of a PAn film. It was found that a PAn film lost its electro-conductivity by the oxidase-HRP reactions, and the oxidized PAn film obtained electro-conductivity again by applying + 100 mV vs. Ag/AgCl to a PAn film.