TY - JOUR
T1 - Effects of Keishi-bukuryo-gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats
AU - Goto, H.
AU - Shimada, Y.
AU - Sekiya, N.
AU - Yang, Q.
AU - Kogure, T.
AU - Mantani, N.
AU - Hikiami, H.
AU - Shibahara, N.
AU - Terasawa, K.
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Funds for Comprehensive Research on Aging and Health from the Japanese Ministry of Health and Welfare. The authors thank Dr. B. Makino and Dr. I. Sakakibara, Tsumura Kampo and Pharmacobnosy Laboratories for measurement of 3D-HPLC finger print.
PY - 2004/2
Y1 - 2004/2
N2 - Keishi-bukuryo-gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-bukuryo-gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-bukuryo-gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation end-product, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-bukuryo-gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO 2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-bukuryo-gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-bukuryo-gan showed a protective effect against vascular injury in the susceptible rat.
AB - Keishi-bukuryo-gan (Gui-zhi-fu-ling-wan) is a formula used for the improvement of blood circulation. Recently it has often also been used for arteriosclerosis. One of the mechanisms involved is thought to be the improvement of endothelial dysfunction, but the details are still unclear. In this study, the effect of Keishi-bukuryo-gan on vascular function and hemorheological factors in spontaneously diabetic (WBN/kob) rats was studied. Rats were given Keishi-bukuryo-gan in chow for 30 weeks. Body weight, blood glucose, endothelium-dependent/-independent relaxation, vasocontraction by free radical-induced and contractive prostanoids, triglyceride, advanced glycation end-product, lipid peroxides, serum NO2-/NO3- and blood viscosity were measured. The results indicated that Keishi-bukuryo-gan caused a decrease in endothelium-dependent relaxation by acetylcholine to become significantly increased, and vasocontraction induced by free radicals and contractive prostanoids was significantly decreased. Furthermore, serum NO 2-/NO3- and blood viscosity were significantly decreased. From these results, it was supposed that Keishi-bukuryo-gan exerted a protective effect on the endothelium. The WBN/kob rat is a useful study model for the complications of human diabetes, and Keishi-bukuryo-gan showed a protective effect against vascular injury in the susceptible rat.
KW - Blood viscosity
KW - Endothelial function
KW - Keishi-bukuryo-gan
KW - NO-/NO-
KW - Spontaneously diabetic (WBN/kob) rats
UR - http://www.scopus.com/inward/record.url?scp=1542288357&partnerID=8YFLogxK
U2 - 10.1078/0944-7113-00336
DO - 10.1078/0944-7113-00336
M3 - 学術論文
C2 - 15070171
AN - SCOPUS:1542288357
SN - 0944-7113
VL - 11
SP - 188
EP - 195
JO - Phytomedicine
JF - Phytomedicine
IS - 2-3
ER -