TY - JOUR
T1 - Dynamic changes in expression of heme oxygenases in mouse heart and liver during hypoxia
AU - Han, Feng
AU - Takeda, Kazuhisa
AU - Yokoyama, Satoru
AU - Ueda, Hideo
AU - Shinozawa, Yotaro
AU - Furuyama, Kazumichi
AU - Shibahara, Shigeki
N1 - Funding Information:
This study was supported in part by Grants-in-Aid for Scientific Research (B), for Scientific Research on Priority Areas, and by the 21st Century COE Program Special Research Grant “the Center for Innovative Therapeutic Development for Common Diseases” from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2005/12/9
Y1 - 2005/12/9
N2 - Heme oxygenase cleaves heme to form biliverdin, carbon monoxide (CO), and iron, and consists of two structurally related isozymes, HO-1 and HO-2. HO-2 is also known as a potential oxygen sensor. Here we show that the relative CO content in arterial blood, which reflects the total amount of endogenous heme degradation, dynamically changes in mice during acclimatization to normobaric hypoxia (10% O2), with the two peaks at 1 day and 21 days of hypoxia. The expression levels of HO-1 and HO-2 proteins were decreased by 20% and 40%, respectively, in the mouse liver at 7 days of hypoxia, which returned to the basal levels at 14 days. On the other hand, HO-1 and HO-2 proteins were increased 2-fold and 1.3-fold, respectively, in the heart at 28 days of hypoxia. Thus, hypoxia induces or represses the expression of HO-1 and HO-2 in vivo, depending on cellular microenvironments.
AB - Heme oxygenase cleaves heme to form biliverdin, carbon monoxide (CO), and iron, and consists of two structurally related isozymes, HO-1 and HO-2. HO-2 is also known as a potential oxygen sensor. Here we show that the relative CO content in arterial blood, which reflects the total amount of endogenous heme degradation, dynamically changes in mice during acclimatization to normobaric hypoxia (10% O2), with the two peaks at 1 day and 21 days of hypoxia. The expression levels of HO-1 and HO-2 proteins were decreased by 20% and 40%, respectively, in the mouse liver at 7 days of hypoxia, which returned to the basal levels at 14 days. On the other hand, HO-1 and HO-2 proteins were increased 2-fold and 1.3-fold, respectively, in the heart at 28 days of hypoxia. Thus, hypoxia induces or represses the expression of HO-1 and HO-2 in vivo, depending on cellular microenvironments.
KW - Bilirubin
KW - Carbon monoxide
KW - Heart
KW - Heme oxygenase
KW - Hypoxia
KW - Liver
KW - Oxygen sensor
UR - http://www.scopus.com/inward/record.url?scp=27544502147&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.08.100
DO - 10.1016/j.bbrc.2005.08.100
M3 - 学術論文
C2 - 16137650
AN - SCOPUS:27544502147
SN - 0006-291X
VL - 338
SP - 653
EP - 659
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -