Drug response analysis for scaffold-free cardiac constructs fabricated using bio-3D printer

Kenichi Arai*, Daiki Murata, Shoko Takao, Anna Nakamura, Manabu Itoh, Takahiro Kitsuka, Koichi Nakayama

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

36 被引用数 (Scopus)

抄録

Cardiac constructs fabricated using human induced pluripotent stem cells-derived cardiomyocytes (iPSCs-CMs) are useful for evaluating the cardiotoxicity of and cardiac response to new drugs. Previously, we fabricated scaffold-free three-dimensional (3D) tubular cardiac constructs using a bio-3D printer, which can load cardiac spheroids onto a needle array. In this study, we developed a method to measure the contractile force and to evaluate the drug response in cardiac constructs. Specifically, we measured the movement of the needle tip upon contraction of the cardiac constructs on the needle array. The contractile force and beating rate of the cardiac constructs were evaluated by analysing changes in the movement of the needle tip. To evaluate the drug response, contractile properties were measured following treatment with isoproterenol, propranolol, or blebbistatin, in which the movement of the needle tip was increased following isoproterenol treatment, but was decreased following propranolol or blebbistain, treatments. To evaluate cardiotoxicity, contraction and cell viability of the cardiac constructs were measured following doxorubicin treatment. Cell viability was found to decrease with decreasing movement of the needle tip following doxorubicin treatment. Collectively, our results show that this method can aid in evaluating the contractile force of cardiac constructs.

本文言語英語
論文番号8972
ジャーナルScientific Reports
10
1
DOI
出版ステータス出版済み - 2020/12/01

ASJC Scopus 主題領域

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