Disruption of CYP88B1 by transcription activator-like effector nuclease in potato and potential use to produce useful saponins

Shuhei Yasumoto*, Hyoung Jae Lee, Ryota Akiyama, Satoru Sawai, Masaharu Mizutani, Naoyuki Umemoto, Kazuki Saito, Toshiya Muranaka

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

1 被引用数 (Scopus)

抄録

Potatoes produce steroidal glycoalkaloids (SGAs), toxic secondary metabolites associated with food poisoning. SGAs are synthesized by multiple biosynthetic enzymes. Knockdown of the CYP88B1 gene, also known as PGA3 or GAME4, is predicted to reduce toxic SGAs and accumulate steroidal saponins. These saponins not only serve as a source of steroidal drugs but are also anticipated to confer disease resistance to potatoes. In this study, we employed transcription activator-like effector nucleases (TALENs) for genome editing to disrupt CYP88B1. We introduced the TALEN expression vector via Agrobacterium-mediated transformation into seven potato lines. In six of these lines, disruption of the CYP88B1 gene was confirmed. Liquid chromatography-mass spectrometry analysis revealed that SGAs were reduced to undetectable levels, corroborating the accumulation of steroidal saponins observed in previous knockdown studies. Our findings demonstrate the feasibility of generating low-toxicity potato lines through CYP88B1 gene disruption using genome editing techniques.

本文言語英語
ページ(範囲)289-293
ページ数5
ジャーナルPlant Biotechnology
41
3
DOI
出版ステータス出版済み - 2024

ASJC Scopus 主題領域

  • バイオテクノロジー
  • 農業および作物学
  • 植物科学

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