TY - GEN
T1 - Detection of single cell activity by using fluorescence-based multiscale pH and oxygen sensors
AU - Suzuki, Masayasu
AU - Yamada, Takeshi
AU - Kato, Satoshi
AU - Iribe, Yasunori
PY - 2008
Y1 - 2008
N2 - This paper reports a parallel monitoring method for single cell metabolic and respiration activity by using a microarrayed pH and oxygen sensor. Optical sensor film was prepared by immobilizing fluorescein isothiocyanate (for pH sensor array) or dichlorotris (1,10-phenanthroline) ruthenium (II) (for oxygen sensor array) onto a diamond like carbon sputtered slide glass. Micro well (10 μm i.d.) array was prepared with carbon black doped PDMS (or epoxy resin) onto the sensor film. The prepared microarrayed pH and oxygen sensor enabled independent pH and oxygen sensing in each micro well, and the difference of metabolic and respiration activity of single cell (mouse lymphocyte) could be detected in a microarrayed pH and oxygen sensor.
AB - This paper reports a parallel monitoring method for single cell metabolic and respiration activity by using a microarrayed pH and oxygen sensor. Optical sensor film was prepared by immobilizing fluorescein isothiocyanate (for pH sensor array) or dichlorotris (1,10-phenanthroline) ruthenium (II) (for oxygen sensor array) onto a diamond like carbon sputtered slide glass. Micro well (10 μm i.d.) array was prepared with carbon black doped PDMS (or epoxy resin) onto the sensor film. The prepared microarrayed pH and oxygen sensor enabled independent pH and oxygen sensing in each micro well, and the difference of metabolic and respiration activity of single cell (mouse lymphocyte) could be detected in a microarrayed pH and oxygen sensor.
UR - http://www.scopus.com/inward/record.url?scp=62449327209&partnerID=8YFLogxK
U2 - 10.1109/MHS.2008.4752469
DO - 10.1109/MHS.2008.4752469
M3 - 会議への寄与
AN - SCOPUS:62449327209
SN - 9781424429196
T3 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008
SP - 311
EP - 316
BT - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
T2 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
Y2 - 6 November 2008 through 9 November 2008
ER -