TY - JOUR
T1 - Constituents of Amoora cucullata with TRAIL resistance-overcoming activity
AU - Ahmed, Firoj
AU - Toume, Kazufumi
AU - Sadhu, Samir K.
AU - Ohtsuki, Takashi
AU - Arai, Midori A.
AU - Ishibashi, Masami
PY - 2010/8/21
Y1 - 2010/8/21
N2 - In search of bioactive natural products for overcoming TRAIL resistance from natural resources, we previously reported a number of active compounds. Bioassay-guided fractionation of mangrove, Amoora cucullata, collected from Sundarbans Mangrove Forest, Bangladesh, led to the isolation of four new compounds (1-4), along with seven known compounds (5-11). Of the isolates, compounds 1, 5, 8, and 9 showed TRAIL resistance-overcoming activity, among which 8 showed the most potent activity and enhanced TRAIL-induced apoptosis in TRAIL-resistant human gastric adenocarcinoma (AGS) cells through the activation of caspase-3/7, enhancing the expression of DR4 and DR5 mRNA in AGS cells. Cell death caused by the combined treatment of 8 and TRAIL was inhibited by human recombinant DR5/Fc and DR4/Fc chimera proteins, indicating that 8 sensitizes TRAIL-resistant AGS cells to TRAIL through the induction of DR4 and DR5.
AB - In search of bioactive natural products for overcoming TRAIL resistance from natural resources, we previously reported a number of active compounds. Bioassay-guided fractionation of mangrove, Amoora cucullata, collected from Sundarbans Mangrove Forest, Bangladesh, led to the isolation of four new compounds (1-4), along with seven known compounds (5-11). Of the isolates, compounds 1, 5, 8, and 9 showed TRAIL resistance-overcoming activity, among which 8 showed the most potent activity and enhanced TRAIL-induced apoptosis in TRAIL-resistant human gastric adenocarcinoma (AGS) cells through the activation of caspase-3/7, enhancing the expression of DR4 and DR5 mRNA in AGS cells. Cell death caused by the combined treatment of 8 and TRAIL was inhibited by human recombinant DR5/Fc and DR4/Fc chimera proteins, indicating that 8 sensitizes TRAIL-resistant AGS cells to TRAIL through the induction of DR4 and DR5.
UR - http://www.scopus.com/inward/record.url?scp=77955121236&partnerID=8YFLogxK
U2 - 10.1039/c004927a
DO - 10.1039/c004927a
M3 - 学術論文
C2 - 20571616
AN - SCOPUS:77955121236
SN - 1477-0520
VL - 8
SP - 3696
EP - 3703
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
IS - 16
ER -