Comprehensive analysis of the functional TCR repertoire at the single-cell level

Tatsuhiko Ozawa, Kazuto Tajiri, Hiroyuki Kishi*, Atsushi Muraguchi

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

26 被引用数 (Scopus)

抄録

A Vβ TCR repertoire is analyzed for understanding the T-cell population in the immune response. However, the TCR repertoire of the Vα-Vβ pair is difficult to analyze because no suitable analytical method is available. Here, we have applied the single-cell 5′-RACE method for amplifying TCR cDNAs from single T-cells and analyzed the repertoire of Vα-Vβ pairs in human T-cells that responded to a superantigen, SEB. We found that the TCR Vβ profile of the SEB-stimulated CD4+ T-cells was in accordance with the previous reports, that the TCR Vα profile also exhibited a prominent difference, and that the TCR Vα-Vβ pairs of the SEB-responding T-cells were promiscuous. We have also found a significant dual TCRα expression in single T-cells. This is the first report of a comprehensive analysis of the functional repertoire of Vα-Vβ pairs at the single T-cell level. This novel method may contribute to TCR-based immunotherapeutics.

本文言語英語
ページ(範囲)820-825
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
367
4
DOI
出版ステータス出版済み - 2008/03/21

ASJC Scopus 主題領域

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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