TY - JOUR
T1 - Cloning and Expression of Putative Rac/Rop GTPase Genes, Am-rac1 and Am-rac2, Involved in Methyl Jasmonate-Induced Transcriptional Activation of Farnesyl Diphosphate Synthase in Cell Cultures of Aquilaria microcarpa
AU - Kenmotsu, Y.
AU - Asano, K.
AU - Yamamura, Y.
AU - Kurosaki, F.
N1 - Funding Information:
Acknowledgments This work was supported, in part, by research grants from the Ministry of Education, Culture, Sports, Science & Technology in Japan, and from Cosmetology Research Foundation.
PY - 2013/6
Y1 - 2013/6
N2 - A homology-based cloning strategy yielded two cDNA clones presumably encoding Rac/Rop GTPases, Am-rac1 and Am-rac2, from callus cultures of Aquilaria microcarpa. Transcriptional levels of Am-rac1, measured using reverse transcription polymerase chain reaction, remained steady, while those of Am-rac2 increased dramatically following treatment of cultured cells with either a yeast extract or methyl jasmonate. These results suggested that Am-rac1 was a constitutively expressed homologue, while Am-rac2 was induced in A. microcarpa cells. Moreover, a farnesyl diphosphate synthase gene of A. microcarpa, Am-FaPS1, was transcriptionally activated in A. microcarpa cells grown in the presence of methyl jasmonate; however, expression levels were markedly lowered in the presence of various signal transduction-related inhibitors involved in Ca2+-, Rap/Rop GTPase-, or ubiquitin-dependent signaling processes. Whereas, expression of Am-FaPS1 was markedly increased, even in the absence of methyl jasmonate in A. microcarpa cells over-expressing Am-rac2. These findings suggested that Rac/Rop GTPase proteins played important roles in jasmonate-induced enhancement of terpenoid metabolism in A. microcarpa.
AB - A homology-based cloning strategy yielded two cDNA clones presumably encoding Rac/Rop GTPases, Am-rac1 and Am-rac2, from callus cultures of Aquilaria microcarpa. Transcriptional levels of Am-rac1, measured using reverse transcription polymerase chain reaction, remained steady, while those of Am-rac2 increased dramatically following treatment of cultured cells with either a yeast extract or methyl jasmonate. These results suggested that Am-rac1 was a constitutively expressed homologue, while Am-rac2 was induced in A. microcarpa cells. Moreover, a farnesyl diphosphate synthase gene of A. microcarpa, Am-FaPS1, was transcriptionally activated in A. microcarpa cells grown in the presence of methyl jasmonate; however, expression levels were markedly lowered in the presence of various signal transduction-related inhibitors involved in Ca2+-, Rap/Rop GTPase-, or ubiquitin-dependent signaling processes. Whereas, expression of Am-FaPS1 was markedly increased, even in the absence of methyl jasmonate in A. microcarpa cells over-expressing Am-rac2. These findings suggested that Rac/Rop GTPase proteins played important roles in jasmonate-induced enhancement of terpenoid metabolism in A. microcarpa.
KW - Aquilaria microcarpa
KW - Farnesyl diphosphate synthase
KW - Methyl jasmonate
KW - Rac/Rop GTPase
KW - Signal transduction
KW - Yeast extract
UR - http://www.scopus.com/inward/record.url?scp=84877818731&partnerID=8YFLogxK
U2 - 10.1007/s11105-012-0529-0
DO - 10.1007/s11105-012-0529-0
M3 - 学術論文
AN - SCOPUS:84877818731
SN - 0735-9640
VL - 31
SP - 539
EP - 546
JO - Plant Molecular Biology Reporter
JF - Plant Molecular Biology Reporter
IS - 3
ER -