Cloning and characterization of a gene encoding Rac/Rop-like monomeric guanosine 5′-triphosphate-binding protein from Scoparia dulcis

Toshiaki Mitamura, Masato Shite, Yoshimi Yamamura, Fumiya Kurosaki*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

5 被引用数 (Scopus)

抄録

A cDNA clone, designated Sd-racrop (969 bp), was isolated from seedlings of Scoparia dulcis. This gene contains an open reading frame encoding the protein of 197 amino acid residues with high homology to Rac/Rop small guanosine 5′-triphosphate-binding proteins from various plant sources. In Southern hybridization analysis, the restriction digests prepared from genomic DNA of S. dulcis showed a main signal together with a few weakly hybridized bands. The transcriptional level of Sd-racrop showed a transient decrease by exposure of the leaf tissues of S. dulcis to the ethylene-generating reagent 2-chloroethylphosphonic acid. However, an appreciable increase in gene expression was reproducibly observed upon treatment of the plant with methyl jasmonate. These results suggest that the Sd-racrop product plays roles in ethylene- and methyl jasmonate-induced responses of S. dulcis accompanying the change in the transcriptional level, however, the cellular events mediated by this protein toward these external stimuli would be regulated by various mechanisms.

本文言語英語
ページ(範囲)1122-1125
ページ数4
ジャーナルBiological and Pharmaceutical Bulletin
32
6
DOI
出版ステータス出版済み - 2009/06

ASJC Scopus 主題領域

  • 薬理学
  • 薬科学

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