Circulating tumor cells detected by the expression of cancer stem cell markers cd90 and cd44 in patients with esophageal cancer

Tomoyuki Okumura*, Tetsuji Yamaguchi, Katsuhisa Hirano, Toru Watanabe, Takuya Nagata, Yutaka Shimada, Kazuhiro Tsukada

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

4 被引用数 (Scopus)

抄録

Background: Epithelial cell adhesion molecule (EpCAM) is a marker for circulating tumor cells (CTCs) in various types of cancer. Cell surface antigens, such as CD90 and CD44, have been reported to be cancer stem cell (CSC) markers in esophageal squamous cell carcinoma (ESCC). The aim of this study was to assess the use of CD90 and CD44 as markers to identify clinically significant CTC subpopulations in ESCC. Methods: We collected 3 mL of peripheral blood from 10 ESCC patients and 10 healthy volunteers to detect combined expression of EpCAM, CD90, and CD40 using flow cytometry. Results: The number of EpCAM-positive cell counts (average 6 SD) in the patients was significantly higher than healthy volunteers (29.1 6 35.9 and 2.3 6 2.5, P 0.001). The proportions (average 6SD) of CD90-and CD44-positive cells in EpCAM-positive cells were 45.7% 6 42.4% and 98.7% 6 2.7%, respectively. EpCAM-positive/CD44-positive CTC counts, which was equivalent to EpCAM-positive CTC counts, correlated with pathologic V factors in the resected primary tumors (P . 0.01). EpCAM-positive/CD90-positive CTC counts, but not EpCAM-positive/CD90-negative CTC counts, correlated with pathologic V factors in the resected primary tumors (P 0.01). Our results suggested that combined expression of EpCAM and CD90 may useful to detect CTC subsets, which have highly metastatic features in ESCC. CD44, on the other hand, is equivalent to EpCAM as a marker to detect CTCs in ESCC.

本文言語英語
ページ(範囲)300-307
ページ数8
ジャーナルInternational Surgery
105
3
DOI
出版ステータス出版済み - 2021

ASJC Scopus 主題領域

  • 外科

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