TY - JOUR
T1 - Chemical cross-talk in flow-type integrated enzyme sensors
AU - Suzuki, Masayasu
AU - Akaguma, Hiroyuki
N1 - Funding Information:
This work was partially supported by Grant-in-Aid for Scientific Research on Priority Areas (No. 296) from the Ministry of Education, Science, Sports and Culture, and by the Research from the Future Program of JSPS.
PY - 2000/6/10
Y1 - 2000/6/10
N2 - In flow-type integrated enzyme sensors, hydrogen peroxide produced at an upstream electrode is transported to downstream electrodes, and causes non-specific responses which are called as "chemical cross talks (CCTs)". In this study, the relationship between CCTs and enzyme immobilization methods, namely an electrochemical pyrrole polymerization, a gelatin-glutaraldehyde crosslinking, and a photocrosslinkable prepolymer (PVA-SbQ) method, was investigated. The flow rate dependency and the substrate concentration dependency of CCTs were dependent on the enzyme immobilization methods. Therefore, the effects of the membrane permeability and enzyme density of bioselective layers on upstream and downstream electrodes, on CCT were investigated by using a gelatin-glutaraldehyde crosslinking method as an enzyme immobilization method. The CCTs correspond to the membrane permeability and enzyme density of a bioselective layer on an upstream electrode. Based on these results, an elimination method for CCT was proposed.
AB - In flow-type integrated enzyme sensors, hydrogen peroxide produced at an upstream electrode is transported to downstream electrodes, and causes non-specific responses which are called as "chemical cross talks (CCTs)". In this study, the relationship between CCTs and enzyme immobilization methods, namely an electrochemical pyrrole polymerization, a gelatin-glutaraldehyde crosslinking, and a photocrosslinkable prepolymer (PVA-SbQ) method, was investigated. The flow rate dependency and the substrate concentration dependency of CCTs were dependent on the enzyme immobilization methods. Therefore, the effects of the membrane permeability and enzyme density of bioselective layers on upstream and downstream electrodes, on CCT were investigated by using a gelatin-glutaraldehyde crosslinking method as an enzyme immobilization method. The CCTs correspond to the membrane permeability and enzyme density of a bioselective layer on an upstream electrode. Based on these results, an elimination method for CCT was proposed.
KW - Chemical cross-talk
KW - Enzyme sensor
KW - Flow analysis
KW - Glucose sensor
KW - Integrated sensor
KW - Lactate sensor
UR - http://www.scopus.com/inward/record.url?scp=0001613961&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(99)00496-7
DO - 10.1016/S0925-4005(99)00496-7
M3 - 会議記事
AN - SCOPUS:0001613961
SN - 0925-4005
VL - 64
SP - 136
EP - 141
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1-3
T2 - Transducers '99 - 10th International Conference on Solid-State Sensors and Actuators
Y2 - 7 June 1999 through 10 June 1999
ER -