Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus

Tatsuhiko Ozawa*, Aishun Jin, Kazuto Tajiri, Masaya Takemoto, Tomoko Okuda, Kimiyasu Shiraki, Hiroyuki Kishi, Atsushi Muraguchi

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

10 被引用数 (Scopus)

抄録

The extra-cellular domain of the influenza virus matrix protein 2 (M2e) is highly conserved between influenza A virus strains compared to hemagglutinin and neuraminidase, and has long been viewed as a potential and universal vaccine target. M2e induces no or only weak and transient immune responses following infection, making it difficult to detect M2e-specific antibodies producing B-cells in human peripheral blood lymphocytes. Recently, using a single-cell manipulation method, immunospot array assay on a chip (ISAAC), we obtained an M2e-specific human antibody (Ab1-10) from the peripheral blood of a healthy volunteer. In this report, we have demonstrate that Ab1-10 reacted not only to seasonal influenza A viruses, but also to pandemic (H1N1) 2009 virus (2009 H1N1) and highly pathogenic avian influenza A virus, and that the antibody-bound M2e of 2009 H1N1 inactivated the virus with high affinity (∼10 -10M). More importantly, it inhibited 2009 H1N1 viral propagation in vitro. These results suggest that Ab1-10 might be a potential candidate for antibody therapeutics for a wide range of influenza A viruses.

本文言語英語
ページ(範囲)283-287
ページ数5
ジャーナルAntiviral Research
91
3
DOI
出版ステータス出版済み - 2011/09

ASJC Scopus 主題領域

  • 薬理学
  • ウイルス学

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